Complement Fixation - Revision history

Bara at 16:48, 17 March 2012

2012-03-17T16:48:39Z

Bara at 21:29, 18 April 2011

2011-04-18T21:29:21Z

Bara at 21:28, 18 April 2011

2011-04-18T21:28:56Z

Bara: moved Complement fixation to Complement Fixation

2011-04-18T21:24:57Z

moved Complement fixation to Complement Fixation

Bara at 14:55, 12 August 2010

2010-08-12T14:55:14Z

A.allison: Text replace - 'Immunology - WikiBlood' to 'Immunology'

2010-06-12T16:08:22Z

Text replace - 'Immunology - WikiBlood' to 'Immunology'

Jtulloch at 08:44, 19 September 2008

2008-09-19T08:44:20Z

Eayton at 12:40, 12 September 2008

2008-09-12T12:40:41Z

Eayton: New page: Complement fixation is a test that exploits the fact that antibody-antigen complexes are able to activate the complement system, using the mechanism to show the presence of a specific anti...

2008-09-12T12:39:05Z

New page: Complement fixation is a test that exploits the fact that antibody-antigen complexes are able to activate the complement system, using the mechanism to show the presence of a specific anti...

New page

Complement fixation is a test that exploits the fact that antibody-antigen complexes are able to activate the complement system, using the mechanism to show the presence of a specific antibody in a serum sample.

===Method===
A mixture of antibody, complement and antigen is incubated and an indicator system (normally antibody-coated sheep red blood cells) is added.
*As the complement reaction with the immune complex produces no visible result, sheep red blood cells are coated with anti-sheep red blood cell antibody. The complement in the mixture will react with this antibody and lyse the cells.
An indication of a positive test is the absence of lysis of the red blood cells, as the complement has already been used up by the antigen-antibody system. Controls are included to ensure that none of the reagents have taken up the complement non-specifically (e.g. contaminated serum)

===Applications===
*'''Wasserman reaction'''- used in the diagnosis of syphilis, the test consists of a mixture of Wasserman antigen, dilutions of the patients serum and complement (normally sourced from guinea pigs).
*Virus detection- tissue samples are innoculated with blood or tissue samples from a patient and tested using complement fixation

← Older revision		Revision as of 16:48, 17 March 2012
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	{{review}}		{{review}}
		+	<br><br>
		+	{{Jim Bee 2007}}
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 *'''Wasserman reaction'''- used in the diagnosis of syphilis, the test consists of a mixture of Wasserman antigen, dilutions of the patients serum and complement (normally sourced from guinea pigs).
 *Virus detection- tissue samples are innoculated with blood or tissue samples from a patient and tested using complement fixation
 {{review}}
 [[Category:Immunological Testing]]

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 {{toplink
 |backcolour = FFE4E1
-|linkpage =Immunology - WikiBlood
+|linkpage =Immunology
 |linktext =IMMUNOLOGY
 |sublink1 =Immunological testing - WikiBlood

← Older revision		Revision as of 08:44, 19 September 2008
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		+	{{review}}
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	{{toplink		{{toplink
	\|backcolour = FFE4E1		\|backcolour = FFE4E1

← Older revision		Revision as of 12:40, 12 September 2008
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−	Complement fixation is a test that exploits the fact that antibody-antigen complexes are able to activate the complement system, using the mechanism to show the presence of a specific antibody in a serum sample.	+	{{toplink
		+	\|backcolour = FFE4E1
		+	\|linkpage =Immunology - WikiBlood
		+	\|linktext =IMMUNOLOGY
		+	\|sublink1 =Immunological testing - WikiBlood
		+	\|subtext1 =IMMUNOLOGICAL TESTING
		+	\|pagetype =Blood
		+	}}
		+
		+
		+	Complement fixation is a test that exploits the fact that antibody-antigen complexes are able
		+	to activate the complement system, using the mechanism to show the presence of a specific antibody in a serum sample.

	===Method===		===Method===

@@ Line 1: / Line 1: @@
-{{review}}
+Also known as: '''''CFT — Complement Fixation Test'''''
 Complement fixation is a test that exploits the fact that antibody-antigen complexes are able
 to activate the complement system, using the mechanism to show the presence of a specific antibody in a serum sample.
-===Method===
+==Method==
 A mixture of antibody, complement and antigen is incubated and an indicator system (normally antibody-coated sheep red blood cells) is added.
 *As the complement reaction with the immune complex produces no visible result, sheep red blood cells are coated with anti-sheep red blood cell antibody. The complement in the mixture will react with this antibody and lyse the cells.
 An indication of a positive test is the absence of lysis of the red blood cells, as the complement has already been used up by the antigen-antibody system. Controls are included to ensure that none of the reagents have taken up the complement non-specifically (e.g. contaminated serum)
-===Applications===
+==Applications==
 *'''Wasserman reaction'''- used in the diagnosis of syphilis, the test consists of a mixture of Wasserman antigen, dilutions of the patients serum and complement (normally sourced from guinea pigs).
 *Virus detection- tissue samples are innoculated with blood or tissue samples from a patient and tested using complement fixation
 [[Category:Immunological Testing]]

← Older revision	Revision as of 21:24, 18 April 2011
(No difference)