Fasciola hepatica

Also known as:

Liver Fluke

Introduction

Fasciola Hepatica is an hepatic parasite found in mainly in ruminants, namely cows, sheep and goats, but also known to affect horses and pigs. It is found Worldwide, and within the UK, with its prevalence ever increasing. It is responsible for a 10-15% production loss in each infected animal, as it affects meat, milk and wool production, so is of huge economic consequence.

Fasciola Hepatica has a definitive ruminant mammalian host and an intermediate molluscan host. Within Europe the intermediate host is almost exclusively the snail 'Lymnaea truncatulata'. The snail habitat is crucial to the survival of the parasite, so wet conditions are favourable to the development and spread of Fasciola hepatica.

Fasciola hepatica (Image sourced from Adam Cuerden, Wikimedia Commons)

Scientific Classification

Kingdom	Animalia
Phylum	Platyhelminthes
Class	Trematoda
Subclass	Digenea
Order	Echinostomida
Family	Fasciolidae
Genus	Fasciola
Species	F. Hepatica

Hosts

Fasciola hepatica has an indirect life cycle, meaning it has both intermediate and final hosts.

Fasciola hepatica is seen most commonly in sheep, cattle, and goats, but may also be seen in horse, deer, and man.

The most important intermediate host within Europe is the snail of the genus Lymnaea. The most common, Lymnaea truncatula, which is an amphibious snail found worldwide.

Life Cycle

Adult flukes in the bile ducts shed eggs directly into the bile, which then subsequently enter the intestine. Eggs are then passed out in the faeces of the mammalian host, where they develop and hatch releasing motile ciliated miracidia. These require 9-10 days at optimal temperatures, of around 22-26 degrees. The miracidium have a short life and must locate a suitable snail, the intermediate host, within approximately 3 hours if they are to be effective and continue the life cycle.

If successful, the miracidium will then develop into sporocysts, then enter the redial stages to the final stages within the intermediate host, which is development into cercaria. These cercaria are then released from the snail, and attach to surfaces such as the tips of grass. Here they encyst and form metacercaria. This represents the infective stage of the lifecycle.

Development from miracidium into metacercariae takes around 6-7 weeks under favourable conditions, however, this period can be much longer in unfavourable conditions.

The final host, or the definitive host then ingests the metacercariae from the grass on the pasture, and these pass through the body into the intestine, where they excyst in the wall of the small intestine. They then travel through the wall of the gut, and migrate into the liver, through the liver parenchyma. The young liver flukes migrate through the liver for around 6-8 weeks before entering the bile ducts. They may also migrate into the gall bladder, where they reach full sexual maturity.

The prepatent period of Fasciola hepatica is 10-12 weeks. In untreated sheep it may survive and continue to infect for many years. In cattle it is usually less than 1 year.

Snail biology

Lymnaea truncatula - Wikimedia Commons

Lymnaea truncatula

• 5-10mm long
• Brown-black shell with 5-6 spirals
• 1st spiral is greater than half the total length
• The shell opens on the right (when held with the opening upwards)
• Feeds on green slime
• Multiplies rapidly when food is abundant
• Most die during the British winter (unless very mild)
• Survivors lay eggs in spring, which hatch in June

Habitats

• Lymnaea are found in muddy areas (but not on highly acidic soils)
• Habitats may be permanent (dry summer) or temporary (wet summer)

Epidemiology

In temperate areas, there are two superimposed epidemiological cycles, known as the summer and winter infections of the snail. On mainland Britain, the summer cycle predominates as a high proportion of snails perish during the winter, but very occasionally, weather sequences allow the winter cycle to affect the pattern of disease. On the west coast of Ireland, the winter cycle of events determines the timing of clinical outbreaks.

Summer infection of the snail

Fluke eggs passed in spring

→ hatch in June (i.e. coincident with snail hatch)

→ miracidia infect newly hatched snails

→ develop and multiply in snail hepatopancreas during summer

→ cercariae shed from late August onwards

→ metacercariae ingested by sheep

→ immature flukes migrate through liver

→ acute disease September-November; or chronic disease January onwards

Winter infection of the snail

Fluke eggs passed in late summer

→ infect snails

→ development halted when temperature <10°C (i.e. flukes trapped in hibernating snails through the winter)

→ development resumes when temperature >10°C

→ cercariae shed from July

→ disease from August

Pathogenesis

The severity of the infection is mainly dependent on the number of metacercariae ingested. The Pathogenesis is often described as two-fold. The first stage occurring when the parasite migrates through the liver parenchyma, causing liver damage and haemorrhage. The second phase occurs when the parasite is in the bile ducts, and damage is a result of the haematophagic activity of the adult flukes.

References

Taylor, M.A, Coop, R.L., Wall,R.L. (2007) Veterinary Parasitology Blackwell Publishing

G.L. Pritchard et al., Emergence of fasciolosis in cattle in East Anglia, The Veterinary Record, November 5, 2005.