Difference between revisions of "Category:Mycobacterium species"

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[[Mycobacterium lepraemurium]]
 
[[Mycobacterium lepraemurium]]
  
 +
[[Mycobacterium avium subsp. paratuberculosis]]
  
  
  
===[[Intestines Proliferative Enteritis - Pathology#Paratuberculosis (Johnes disease)|Johne's Disease (paratuberculosis)]]===
 
 
*[[Intestines Proliferative Enteritis - Pathology#Paratuberculosis (Johnes disease)|Johne's Disease]] is a chronic, contagious enteritis of ruminants
 
*Caused by ''M avium'' subsp. ''paratuberculosis''
 
 
*Epidemiology
 
**Transmitted to young calves by ingestion of mycobacteria in faeces of infected adults
 
**Organisms viable in environment for long periods
 
**Long incubation period with clinical signs appearing in cattle over 2 years of age
 
**Subclinical carriers can occur, shedding organisms in their faeces
 
 
*Pathogenesis and pathogenicity
 
**''M avium'' subsp. ''paratuberculosis'' is an intracellular pathogen
 
**Mycobacteria are ingested by macrophages in the Peyer's patches
 
**Survival and replication of mycobacteria in macrophages initiate an immune-mediated granulomatous reaction
 
**Lymphocytes and macrophages accumulate  in the lamina propria and submucosa, resulting in marked thickening and folding of the intestinal wall
 
**Mesenteric lymph nodes are enlarged
 
**A protein-losing enteropathy results, along with failure to absorb nutrients and water
 
 
*Clinical signs
 
**Diarrhoea, initially intermittent, and weight loss in cattle
 
**Weight loss in sheep and goats
 
**Rapidly fatal with weight loss and diarrhoea in some deer
 
 
*Diagnosis
 
**All diagnostic procedures have faults but include:
 
**Microscopy of rectal biopsies
 
**Faecal culture
 
**Serology of serum including complement fixation tests, agar-gel immunodiffusion test and an ELISA
 
**Histopathology of intestines and lymph nodes
 
**Isolation and identification of mycobacteria from faeces and tissues
 
**Ziehl-Neelson-positive smears
 
**Intradermal tuberculin test
 
**DNA probes for detection in faeces
 
 
*Control
 
**Slaughter of affected animals
 
**Detection and slaughter of subclinical shedders using faecal culture, DNA probes and ELISA
 
**Good hygiene to protect young calves
 
**Separation and isolation of calves from affected dams
 
**Inactivated adjuvanted vaccines are available and reduce shedding of mycobacteria but do not eliminate infection
 
 
[[Category:Bacteria]][[Category:Gram_positive_bacteria]][[Category:Rods]]
 
[[Category:Bacteria]][[Category:Gram_positive_bacteria]][[Category:Rods]]

Revision as of 10:59, 14 May 2010


Overview

  • Mycobacterial infections are caused by bacteria belonging to the family Mycobacteriaceae, order Actinomycetales
  • Includes obligate pathogens, opportunistic pathogens and saprophytes
  • Cause chronic, progressive, granulomatous infections
  • Cause tuberculosis, Johne's disease and feline leprosy
  • M. bovis, M. tuberculosis and M. avium cause tuberculosis of cattle, tuberculosis of pigs and tuberculosis of dogs respectively
  • The 'classical' tuberculosis lesions are caused by the Mycobacterium tuberculosis complex
  • The Johne's type lesions are caused by the Mycobacterium avium complex
  • Environmental species are found in soil, vegetation and water
  • Mycobacterium leprae and M.lepraemurium cause human, feline/murine leprosy respectively
  • Atypical mycobacteriosis is a localized opportunistic skin and subcutaneous infection caused by saprophytic and rapidly growing atypical mycobacteria
  • Granulomatous lesions in muscle and skin
  • Skin tuberculosis of cattle causes nodules along the lymphatics of the limbs
  • Bovine farcy is thought to be caused by M. senegalense and M. farcinogenes
  • Saprophytic mycobacteria cause granulomatous lesions

Characteristics

  • Aerobic, weakly Gram-positive acid-fast rods
  • Non-motile, non-spore forming
  • Cell walls contain mycolic acid
  • Require egg-based media for growth
  • Slow-growing colonies
  • Resistant to disinfectants and environmental conditions; susceptible to pasteurisation
  • Mycobacteria stain with carbol dyes and resist subsequent decolorization with inorganic acids; this characteristic which is due to the spatial arrangement of mycolic acids within the cell wall makes them acid fast


Identification

  • Identified by Ziehl-Neelson staining
  • Differentiated by culture, biochemical tests, chromatography and molecular techniques
  • Pathogenic species require at least three weeks for growth on egg-based media

Mycobacterium bovis

Mycobacterium avium

Mycobacterium lepraemurium

Mycobacterium avium subsp. paratuberculosis

Pages in category "Mycobacterium species"

The following 4 pages are in this category, out of 4 total.