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===Bacterial Culture===

===Bacterial Culture===

As  noted above, the selection of the culture medium, atmospheric  conditions and pH are among many variables that need to be considered  for the successful culture of bacteria in the lab. A routine culture  undertaken involves using a combination of blood agar (see below) and  MacConkey agar (see below) together with incubation for between  24-48hours. Blood agar is able top support most pathogenic species of  bacteria and is usually appropriate for routine primary isolation.  Selective media is then normally used for particular organisms.

As  noted above, the selection of the culture medium, atmospheric  conditions and pH are among many variables that need to be considered  for the successful culture of bacteria in the lab. A routine culture  undertaken involves using a combination of blood agar (see below) and  MacConkey agar (see below) together with incubation for between  24-48hours. Blood agar is able to support most pathogenic species of  bacteria and is usually appropriate for routine primary isolation.  Selective media is then normally used for particular organisms.

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The  most common molecular technique used to identify species of pathogenic  bacteria are nucleic acid hybridisation and polymerase chain reactions  (PCR). Nucleic acid hybridisation uses synthetic nucleic acid probes  (specific for a particular species) that are applied to genetic material  extracted from the pathogen. Probes can be used to detect DNA and RNA.  

The  most common molecular technique used to identify species of pathogenic  bacteria are nucleic acid hybridisation and polymerase chain reactions  (PCR). Nucleic acid hybridisation uses synthetic nucleic acid probes  (specific for a particular species) that are applied to genetic material  extracted from the pathogen. Probes can be used to detect DNA and RNA.  

[[Category:Bacteria - Overview]]

 

 

[[Category:Bacteria|D]]