Difference between revisions of "Category:Staphylococcus species"

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*Oxidase-negative
 
*Oxidase-negative
  
===Detections & Differentiation===
+
===Detection & Differentiation===
The most simple method of detection is to gram stain a sample, usually puss, and to then visualise the distinctive clusters under a microscope. This provides a basic identification of the type of bacteria, however occasionally it is necessary to identify the species that causing the infection. This can be done using various techniques based on the different characteristics of the species. Growing cultures on blood agar will enable identification of haemolytic vs. non haemolytic species. PCR is the most specific method of determining the species of bacteria but requires laboratory analysis of samples.
+
The most simple method of detection is to gram stain a sample, usually puss, and to then visualise the distinctive clusters under a microscope. This provides a basic identification of the type of bacteria, however occasionally it is necessary to identify the species that causing the infection. This can be done using various techniques based on the different characteristics of the species. Selective agar such as blood, for haemolysin producing species such as ''S. aureus'' and ''S. intermedius'' as well as non haemolytic species such as ''S. hyicus''. MacConkey agar can be used to identify. PCR is the most specific method of determining the species of bacteria but requires laboratory analysis of samples and is only usually used in research.
  
*''S. aureus'' and ''S. intermedius'' produce double haemolysis; they produce alpha-haemolysin and beta-haemolysin
 
*''S. hyicus'' is non-haemolytic
 
 
*Coagulase-negative strains vary in ability to cause haemolysis
 
*Coagulase-negative strains vary in ability to cause haemolysis
 
*Slide test to detect bound coagulase (clumping factor) on surface of bacteria; bacteria clump within 1-2 minutes
 
*Slide test to detect bound coagulase (clumping factor) on surface of bacteria; bacteria clump within 1-2 minutes
 
*Tube test detects free coagulase, secreted by bactera; positive result indicated by clot formation in tube following 24-hour incubation; free coagulase converts prothrombin to thrombin which converts fibrinogen to fibrin
 
*Tube test detects free coagulase, secreted by bactera; positive result indicated by clot formation in tube following 24-hour incubation; free coagulase converts prothrombin to thrombin which converts fibrinogen to fibrin
*Biochemical tests to differentiate ''S. aureus'' and ''S. intermedius''
 
*PCR to differentiate species
 
  
 
===Pathogenesis and pathogenicity===
 
===Pathogenesis and pathogenicity===

Revision as of 22:00, 13 July 2010


Overview

There are a large number of Staphyloccocus spp present in the environment and many are commensals of the skin and mucous membranes of animals and man. Many are opportunistic pathogens causing pyogenic infections as a result of trauma, immunosuppression and secondary to other infections.

  • Enodgenous or exogenous infections
  • Coagulase-positive S. aureus and S. intermedius as well as S. hyicus are important pathogens of animals
  • Fairly stable in environment
  • Strains selective for particular species
  • Cause mastitis, tick pyaemia, exudative epidermitis, botryomycosis and pyoderma

Characteristics

Although individual species may vary slightly, most have similar characteristics.

  • Gram-positive
  • Form grape-like clusters
  • Moderate sized, white/golden colonies
  • Facultative anaerobes
  • Grow on non-enriched media
  • Non motile
  • Catalase-positive
  • Haemolytic, 4 haemolysins recognised
  • Oxidase-negative

Detection & Differentiation

The most simple method of detection is to gram stain a sample, usually puss, and to then visualise the distinctive clusters under a microscope. This provides a basic identification of the type of bacteria, however occasionally it is necessary to identify the species that causing the infection. This can be done using various techniques based on the different characteristics of the species. Selective agar such as blood, for haemolysin producing species such as S. aureus and S. intermedius as well as non haemolytic species such as S. hyicus. MacConkey agar can be used to identify. PCR is the most specific method of determining the species of bacteria but requires laboratory analysis of samples and is only usually used in research.

  • Coagulase-negative strains vary in ability to cause haemolysis
  • Slide test to detect bound coagulase (clumping factor) on surface of bacteria; bacteria clump within 1-2 minutes
  • Tube test detects free coagulase, secreted by bactera; positive result indicated by clot formation in tube following 24-hour incubation; free coagulase converts prothrombin to thrombin which converts fibrinogen to fibrin

Pathogenesis and pathogenicity

  • Cause suppurative lesions
  • Trauma or immunosuppression predispose to infection
  • Capsular polysaccharide, teichoic acids and potein A prevent opsonisation and therefore phagocytosis
  • Cell wall proteins bind fibronectin and fibrinogen, allowing bacteria to attched to damaged tissues
  • Coagulase, DNase and protein A production mark pathogenicity
  • Haemolysins made by S. aureus and S. intermedius act as toxins
  • Alpha-haemolysin prouces a narrow zone of complete haemolysis; this toxin causes necrosis and targets mast cells and plasma cells, whose contents cause damage
  • Beta-haemolysin produces a wide zone of incomplete haemolysis; damages membranes and causes leakage, contributing to necrosis
  • Also gamma and delta toxins
  • Toxic shock syndrome toxin acts as a superantigen, which causes T cell proliferation and production of cytokines, leading to cardiovascular shock, with microthrombus formation in capillaries
  • Enterotoxins produced by some strains of S. aureus cause food poisoning in humans
  • Proteases, hyaluronidases and lipases facilitate survival of bacteria and spread and tissue destruction

Diagnosis

  • Clusters of bacteria in Gram-stained smears of pus
  • Culture on selective blood agar and MacConkey agar
  • No growth on MacConkey
  • Colony characteristics, haemolysis, catalase and coagulase production
  • Phage typing for epidemiological studies

Infections in dogs and cats

  • S. intermedius causes deep pyoderma, otitis externa, mastitis, endometritis, cystitis, osteomyelitis, wound infections, dyscospondylitis
  • S. aureus may cause gastroenteritis

Coagulase-negative staphylococci

  • Usually harmless commensals or secondary invaders
  • May adhere to indwelling catheters leading to urinary tract infections
  • Often display multiple antibiotic resistance

Enteritis

  • 30% strains of Staphylococcus aureus produce potent enterotoxin. T
    • Protein and heat-stable
    • Responsible for staphylococcal food poisoning in man.
  • Every reason to assume that acute gastro-intestinal disturbance in small animals may be caused by these enterotoxins BUT not well documented.
  • Symptoms last 24-36 hours and include:
    • Acute vomiting
    • Diarrhoea
    • Pain
  • The enterotoxins are superantigens.
    • Induce release of cytokines from lymphocytes



sheep

  • Staphylococci
    • Sporadically infect joints
    • May complicate 'tick-borne fever'


pigs

    • Cause purulent arthritis
    • In one or more joints
    • Commonly contaminate wounds


dogs

    • May cause discospondylitis
    • Destructive inflammatory lesion
    • Bacterial localise in annulus fibrosis -> may spread to local bone

Pages in category "Staphylococcus species"

The following 3 pages are in this category, out of 3 total.