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==Introduction==
==Introduction==
[[Image:ELISA.jpg|thumb|right|150px|Double Antibody Sandwich ELISA]]
[[Image:ELISA.jpg|thumb|right|150px|Double Antibody Sandwich ELISA]]
*Detection and identification of disease agents, e.g. type and subtype
*Detection and identification of disease agents, e.g. type and subtype
*Identification of specific antibodies, e.g. used in serodiagnosis for epidemiological studies
*Identification of specific antibodies, e.g. used in serodiagnosis for epidemiological studies
*Quantification of specific antibody isotypes, e.g. IgM/IgG ratio
*Quantification of specific antibody isotypes, e.g. [[IgM]]/[[IgG]] ratio
==Techniques==
==Techniques==
# Sample serum is added- class-specific antibody in the serum binds to antiglobulin
# Sample serum is added- class-specific antibody in the serum binds to antiglobulin
# Wash
# Wash
# Antigen is added- attaches to specific antibody
# Antigen is added - attaches to specific antibody
# Wash
# Wash
# Enzyme-labeled antibody is added
# Enzyme-labeled antibody is added
# Enzyme substrate is added
# Enzyme substrate is added
''visualised product = amount of enzyme-labelled antigen''
''visualised product = amount of enzyme-labelled antigen''
{{review}}
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{{Jim Bee 2007}}
[[Category:Immunological Testing]]
[[Category:Image Review]]