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Embryo Transfer - Anatomy & Physiology (view source)
Revision as of 13:48, 5 July 2012
, 13:48, 5 July 2012→Transfer of Viable Embryos into Synchronised Recipients
== Introduction ==
== Introduction ==
== Synchronization of Recipients with the Donor ==
== Synchronization of Recipients with the Donor ==
* GOAL: To synchronize the donor and recipient to be in the same stage of the [[Female_Reproductive_Tract_-Oestrous_Cycle_-_Anatomy_%26_Physiology|oestrous cycle]].
* GOAL: To synchronize the donor and recipient to be in the same stage of the [[Oestrous Cycle - Anatomy & Physiology|oestrous cycle]].
* REASON: To prepare the [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|uterus]] of the recipient to support [[Blastocyst Embryonic Development - Anatomy & Physiology|preattachment embryogenesis]].
* REASON: To prepare the [[Uterus - Anatomy & Physiology|uterus]] of the recipient to support [[Blastocyst Embryonic Development - Anatomy & Physiology|preattachment embryogenesis]].
* HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.
* HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.
== Superovulation of the Donor ==
== Superovulation of the Donor ==
* GOAL: To hyperstimulate [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|ovaries]] with gonadotrophins.
* GOAL: To hyperstimulate [[Ovary - Anatomy & Physiology|ovaries]] with gonadotrophins.
* REASON: To provide higher than normal numbers of [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicles]] that reach dominance and [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulate]].
* REASON: To provide higher than normal numbers of [[Follicles - Anatomy & Physiology|follicles]] that reach dominance and [[Ovulation - Anatomy & Physiology|ovulate]].
* HOW: Inject donor with gonadotrophins to hyperstimulate follicular development. Generally '''FSH''' or one of its analogs is used.
* HOW: Inject donor with gonadotrophins to hyperstimulate follicular development. Generally '''FSH''' or one of its analogs is used.
== Inseminating the Donor ==
== Inseminating the Donor ==
* GOAL: Inseminate donor with semen from genetically superior bulls. To generate the best [[Fertilisation%2C_Implantation_and_Early_Embryonic_Development_-Fertilisation-_Anatomy_%26_Physiology|fertilisation]] rates and genetic combinations possible.
* GOAL: Inseminate donor with semen from genetically superior bulls. To generate the best [[Fertilisation - Anatomy & Physiology|fertilisation]] rates and genetic combinations possible.
* REASON: Enhance rate of genetic progress.
* REASON: Enhance rate of genetic progress.
* HOW: Use highly fertile semen and well-trained, experienced inseminators.
* HOW: Use highly fertile semen and well-trained, experienced inseminators.
* REASON: To recover viable embryos.
* REASON: To recover viable embryos.
* HOW:
* HOW:
** Before the procedure is started, a local anaesthetic is injected to cause relaxation of the [[Rectum_-_Anatomy_%26_Physiology|rectum]].
** Before the procedure is started, a local anaesthetic is injected to cause relaxation of the [[Rectum_- Anatomy & Physiology|rectum]].
** At day 6-8 a specialized catheter has a small balloon that can be inflated to prevent retrogade flow of the flushing medium.
** At day 6-8 a specialized catheter has a small balloon that can be inflated to prevent retrogade flow of the flushing medium.
** A flushing medium is then introduced into the [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|uterus]], lavaged and returned through the catheter to a collection vessel.
** A flushing medium is then introduced into the [[Uterus - Anatomy & Physiology|uterus]], lavaged and returned through the catheter to a collection vessel.
* REASON: To achieve pregnancy in each recipient.
* REASON: To achieve pregnancy in each recipient.
* HOW:
* HOW:
** Both donor and recipient have a [[The_Ovary_-Corpus_Luteum_-_Anatomy_%26_Physiology|corpus luteum]] at similar stages of leutinization.
** Both donor and recipient have a [[Corpus Luteum - Anatomy & Physiology|corpus luteum]] at similar stages of leutinization.
** [[Female_Reproductive_Tract_-The_Uterus_-_Anatomy_%26_Physiology|Uterine environment]] in the donor and recipient are quite similar.
** [[Uterus - Anatomy & Physiology|Uterine environment]] in the donor and recipient are quite similar.
** A single embryo is placed into the uterine horn using a transfer pipette.
** A single embryo is placed into the uterine horn using a transfer pipette.
[[Category:Reproductive System]]
[[Category:Reproductive Technologies]]
[[Category:Bullet Points]]