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Also known as: '''''EEV — Alphavirus — Eastern Equine Encephalitis Virus, EEE — Western Equine Encephalitis Virus, WEE — Venezuelan Equine Encephalitis Virus, VEE

| Also known as:

| '''EEV<br>

'''Alphavirus<br>

'''Eastern Equine Encephalitis Virus, EEE<br>

'''Western Equine Encephalitis Virus, WEE<br>

'''Venezuelan Equine Encephalitis Virus, VEE'''

==Introduction==

==Introduction==

'''Genus ''Alphavirus'''''

'''Genus ''Alphavirus'''''

Single-stranded, linear, positive-sense RNA viruses, 60-70nm in diameter.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>[[Image:Alphavirus.gif|300px|thumb|right|''' (Surface of an Alphavirus.  This image is a computer-generated model of the surface of an alphavirus derived by cryoelectron microscopy. The spike-like structures on the virion surface are trimers composed of heterodimers of the virion surface glycoproteins E1 and E2. These spikes are used by the virus to attach to susceptible animal cells.  ''Sourced from Wikimedia Commons, Copyright of the Centers for Disease Control and Prevention (CDC) Division of Vector-Borne Infectious Diseases, 2007)'' ''']]

Single-stranded, linear, positive-sense RNA viruses, 60-70nm in diameter.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>[[Image:Alphavirus.gif|300px|thumb|right|''Surface of an Alphavirus'' JoelMills 2007, Wikimedia Commons]]

===Important Serotypes===

===Important Serotypes===

*Western EEV: ''Culex tarsalis''

*Western EEV: ''Culex tarsalis''

*Venezuelan EEV: ''Culex melanconium'', ''Aedes'' spp., ''Phosphora'' spp.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>.

*Venezuelan EEV: ''Culex melanconium'', ''Aedes'' spp., ''Phosphora'' spp.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>.

''Culiseta melanura'' is another vector for Eastern EEV.  It feeds mostly on swamp birds, completing an enzootic cycle of viral transmission.  ''C.melanura'' is thus an inhabitant of freshwater swamps and is not usually found in areas densely populated by equids.<ref> Hoff, G.L, Bigler, W.J, Buff, E.E, Beck, E (1978) Occurrence and distribution of western equine encephalomyelitis in Florida, ''J Am Vet Med Assoc'', 172:351-352.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Epizootics and epidemics of Eastern EEV disease are propagated by ''Aedes'' spp.  Western EEV persists in an enzootic cycle with passerine birds, transmitted by ''C.tarsalis''.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>.  Other vectors or overwintering hosts for this serotype may include [[Dermacentor spp.|''Dermacentor andersoni'']] ticks<ref> Syverton, J.T, Berry, G.P (1937) The tick as a vector for the virus disease equine encephalomyelitis, J Bacteriol, 33:60.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, ''Triatoma sanguisuga'' (the assassin bug)<ref> Kitselman, C.H, Grundman, A.W (1940) Equine encephalomyelitis virus isolated from naturally infected ''Triatoma sanguisuga'', ''Kans Agric Exp Station Tech Bull'', 50:15.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, and the cliff swallow bug (''Oeciacus vicarius'').<ref> Hayes, R.O, Francy, D.B, Lazuick, J.S (1977) Role of the cliff swallow bug (''Oeciacus vicarius'') in the natural cycle of a Western equine encephalitis-related alphavirus, ''J Entomol'', 14:257-262.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>  Epidemic strains of Venezuelan EEV have infected mosquito species from several genera and this viral serotype may also be transmitted by ticks.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>.

''Culiseta melanura'' is another vector for Eastern EEV.  It feeds mostly on swamp birds, completing an enzootic cycle of viral transmission.  ''C.melanura'' is thus an inhabitant of freshwater swamps and is not usually found in areas densely populated by equids.<ref> Hoff, G.L, Bigler, W.J, Buff, E.E, Beck, E (1978) Occurrence and distribution of western equine encephalomyelitis in Florida, ''J Am Vet Med Assoc'', 172:351-352.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref> Epizootics and epidemics of Eastern EEV disease are propagated by ''Aedes'' spp.  Western EEV persists in an enzootic cycle with passerine birds, transmitted by ''C.tarsalis''.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>.  Other vectors or overwintering hosts for this serotype may include [[Dermacentor spp.|''Dermacentor andersoni'']] ticks<ref> Syverton, J.T, Berry, G.P (1937) The tick as a vector for the virus disease equine encephalomyelitis, ''J Bacteriol'', 33:60.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, ''Triatoma sanguisuga'' (the assassin bug)<ref> Kitselman, C.H, Grundman, A.W (1940) Equine encephalomyelitis virus isolated from naturally infected ''Triatoma sanguisuga'', ''Kans Agric Exp Station Tech Bull'', 50:15.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>, and the cliff swallow bug (''Oeciacus vicarius'').<ref> Hayes, R.O, Francy, D.B, Lazuick, J.S (1977) Role of the cliff swallow bug (''Oeciacus vicarius'') in the natural cycle of a Western equine encephalitis-related alphavirus, ''J Entomol'', 14:257-262.  In: Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>  Epidemic strains of Venezuelan EEV have infected mosquito species from several genera and this viral serotype may also be transmitted by ticks.<ref name="multiple">Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) '''Equine Internal Medicine''' (Third Edition), ''Saunders'', Chapter 12</ref>.

==Virus Identification==

==Virus Identification==

*The complement fixation test can be used to identify Eastern or Western EEV in infected mouse or chicken brains, cell culture fluid or amniotic-allantoic fluid.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>

*The complement fixation test can be used to identify Eastern or Western EEV in infected mouse or chicken brains, cell culture fluid or amniotic-allantoic fluid.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>

*Virus may be identified in brain tissue or cell culture using direct immunofluorescent staining.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>

*Virus may be identified in brain tissue or cell culture using direct immunofluorescent staining.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>

*EEE and WEE viral RNA in mosquitoes and equine tissues may be detected by reverse-transcription PCR.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>   

*EEE and WEE viral RNA in mosquitoes and equine tissues may be detected by reverse-transcription PCR.<ref>Vodkin, M.H, McLaughlin, G.L, Day, J.F, Shope, R.E, Novak, R.J (1993) A rapid diagnostic assay for eastern equine encephalomyelitis viral-RNA, ''Am J Trop Med Hyg'', 49, 772-776.  In: ''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>   

*ELISA can be used to detect virus in brain tissue.  An antigen-capture ELISA, developed for EEE surveillance in mosquitoes, can be used where virus isolation and PCR facilities are unavailable. <ref>Brown, T.M, Mitchell, C.J, Nasci, R.S, Smith, G.C. and Roehrig, J.T. (2001). Detection of eastern equine encephalitis virus in infected mosquitoes using a monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay, ''Am. J. Trop. Med. Hyg.'', 65, 208-213.  In: ''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>

*ELISA can be used to detect virus in brain tissue.  An antigen-capture ELISA, developed for EEE surveillance in mosquitoes, can be used where virus isolation and PCR facilities are unavailable. <ref>Brown, T.M, Mitchell, C.J, Nasci, R.S, Smith, G.C. and Roehrig, J.T. (2001). Detection of eastern equine encephalitis virus in infected mosquitoes using a monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay, ''Am J Trop Med Hyg'', 65, 208-213.  In: ''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>

*Virus isolation is the most definitive diagnostic method for EEE or WEE.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Brain is preferred, but virus has also been isolated from the liver and spleen.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Samples of these tissues should be taken in duplicate, one set for virus isolation and the other placed in formalin for histopathology.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Viral isolation specimens should be sent frozen unless they can be received refrigerated within 48 hours of sampling.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Unless clinical signs persist for more than 5days prior to death, EEE virus is frequently isolated from equine brain tissue.  WEE virus, however, is rarely isolated from tissues of infected horses.  Newborn mice, chicken embryos and a number of cell culture systems can be used for virus isolation.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Virus may also be isolated from cerebrospinal fluid (CSF) of acutely infected horses.<ref name="duplicate">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref>

*Virus isolation is the most definitive diagnostic method for EEE or WEE.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Brain is preferred, but virus has also been isolated from the liver and spleen.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Samples of these tissues should be taken in duplicate, one set for virus isolation and the other placed in formalin for histopathology.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>. Viral isolation specimens should be sent frozen unless they can be received refrigerated within 48 hours of sampling.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Unless clinical signs persist for more than 5days prior to death, EEE virus is frequently isolated from equine brain tissue.  WEE virus, however, is rarely isolated from tissues of infected horses.  Newborn mice, chicken embryos and a number of cell culture systems can be used for virus isolation.<ref name="again">''Manual of Diagnostic Tests and Vaccines for Terrestrial Animals'' found at http://www.oie.int/eng/normes/mmanual/A_00081.htm, accessed July 2010.</ref>.  Virus may also be isolated from cerebrospinal fluid (CSF) of acutely infected horses.<ref name="duplicate">Merck & Co (2008) The Merck Veterinary Manual (Eighth Edition), Merial found at http://www.merckvetmanual.com/mvm/index.jsp?cfile=htm/bc/100900.htm&word=Equine%2cencephalitis, accessed July 2010</ref>

==References==

==References==

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[[Category:Togaviridae]]

[[Category:Togaviridae]]

 

[[Category:Expert_Review]]

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