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| − | == Introduction ==
| + | <big><center>[[Reproductive System|'''BACK TO REPRODUCTIVE SYSTEM''']]</center></big> |
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| − | Multiple ovulation and embryo transfer has been commercially available since the 1970s, but not widely used because it requires a highly skilled team.
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| − | == Synchronization of Recipients with the Donor ==
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| − | * GOAL: To synchronize the donor and recipient to be in the same stage of the [[Oestrous Cycle - Anatomy & Physiology|oestrous cycle]].
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| − | * REASON: To prepare the [[Uterus - Anatomy & Physiology|uterus]] of the recipient to support [[Blastocyst Embryonic Development - Anatomy & Physiology|preattachment embryogenesis]].
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| − | * HOW: Treat recipient with a hormonal regime that induces oestrus to occur at the same time as the donor.
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| − | == Superovulation of the Donor ==
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| − | * GOAL: To hyperstimulate [[Ovary - Anatomy & Physiology|ovaries]] with gonadotrophins.
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| − | * REASON: To provide higher than normal numbers of [[Follicles - Anatomy & Physiology|follicles]] that reach dominance and [[Ovulation - Anatomy & Physiology|ovulate]].
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| − | * HOW: Inject donor with gonadotrophins to hyperstimulate follicular development. Generally '''FSH''' or one of its analogs is used.
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| − | == Inseminating the Donor ==
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| − | * GOAL: Inseminate donor with semen from genetically superior bulls. To generate the best [[Fertilisation - Anatomy & Physiology|fertilisation]] rates and genetic combinations possible.
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| − | * REASON: Enhance rate of genetic progress.
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| − | * HOW: Use highly fertile semen and well-trained, experienced inseminators.
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| − | == Recovery and Identification of Viable Embryos ==
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| − | * GOAL: To nonsurgically collect (flush) embryos from the donor to transfer.
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| − | * REASON: To recover viable embryos.
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| − | * HOW:
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| − | ** Before the procedure is started, a local anaesthetic is injected to cause relaxation of the [[Rectum_- Anatomy & Physiology|rectum]].
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| − | ** At day 6-8 a specialized catheter has a small balloon that can be inflated to prevent retrogade flow of the flushing medium.
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| − | ** A flushing medium is then introduced into the [[Uterus - Anatomy & Physiology|uterus]], lavaged and returned through the catheter to a collection vessel.
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| − | == Transfer of Viable Embryos into Synchronised Recipients ==
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| − | * GOAL: To deposit a potentially viable embryo into the uterine horn of each recipient.
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| − | * REASON: To achieve pregnancy in each recipient.
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| − | * HOW:
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| − | ** Both donor and recipient have a [[Corpus Luteum - Anatomy & Physiology|corpus luteum]] at similar stages of leutinization.
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| − | ** [[Uterus - Anatomy & Physiology|Uterine environment]] in the donor and recipient are quite similar.
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| − | ** A single embryo is placed into the uterine horn using a transfer pipette.
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| − | [[Category:Reproductive Technologies]]
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| − | [[Category:Bullet Points]]
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