Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
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+ | <big><center>[[Reproductive System|'''BACK TO REPRODUCTIVE SYSTEM''']]</center></big> | ||
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+ | <big><center>[[Reproductive_System#Reproductive_Technologies|'''BACK TO REPRODUCTIVE TECHNOLOGIES''']]</center></big> | ||
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== Oocyte Collection== | == Oocyte Collection== | ||
− | * Donor is superovulated to provide large numbers of mature | + | * Donor is superovulated to provide large numbers of mature follicles. |
− | * Pre-ovulatory | + | * Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content. |
− | * | + | * Oocytes captured in vitro. |
− | * Direct [[ | + | * Direct [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] aspiration: |
− | ** A hyperdermic needle is inserted into the [[ | + | ** A hyperdermic needle is inserted into the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]]. |
** Follicular fluid is aspirated. | ** Follicular fluid is aspirated. | ||
− | ** Follicular fluid forcefully returned to the [[ | + | ** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]]. |
− | ** Repeated 2-3 times to dislodge | + | ** Repeated 2-3 times to dislodge oocytes. |
− | + | ||
* Transvaginal Aspiration in the Mare: | * Transvaginal Aspiration in the Mare: | ||
− | ** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_- | + | ** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]]. |
− | ** Lubricated ultrasound transducer is inserted into the [[ | + | ** Lubricated ultrasound transducer is inserted into the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vagina]] and held in the fornix vagina. |
− | ** | + | ** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized. |
− | ** Hypodermic needle is advanced through the [[ | + | ** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle. |
− | ** Follicular fluid containing the | + | ** Follicular fluid containing the oocyte is aspirated under constant vacuum. |
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− | + | * Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa. | |
− | + | ** Could also be cultured in a 'nurse animal' in vivo. | |
− | * | ||
− | ** | ||
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* Embryos are incubated for a further period to the appropriate stage for transfer. | * Embryos are incubated for a further period to the appropriate stage for transfer. | ||
* Embryos transferred to recipient female. | * Embryos transferred to recipient female. | ||
− | * Can be transferred at the correct stage of the recipient's | + | * Can be transferred at the correct stage of the recipient's oestrous cycle. |
− | * Embryos transferred around the [[ | + | * Embryos transferred around the [[Fertilisation%2C_Implantation_and_Early_Embryonic_Development_-Embryonic_Development_of_the_Blastocyst-_Anatomy_%26_Physiology|blastocyst]] stage. |
* Success rates are improving. | * Success rates are improving. | ||
− | * Embryos can be frozen, eliminating the need for tight | + | * Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations. |
− | * Increases incidence of [[Large_Offspring|large offspring syndrome]]. | + | * Increases incidence of [[Parturition_-_Risks_in_Parturition_-_Anatomy_%26_Physiology#Large_Offspring|large offspring syndrome]]. |
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Revision as of 07:39, 17 July 2008
Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
- Oocytes captured in vitro.
- Direct follicle aspiration:
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.
- Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
- Could also be cultured in a 'nurse animal' in vivo.
- Embryos are incubated for a further period to the appropriate stage for transfer.
- Embryos transferred to recipient female.
- Can be transferred at the correct stage of the recipient's oestrous cycle.
- Embryos transferred around the blastocyst stage.
- Success rates are improving.
- Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
- Increases incidence of large offspring syndrome.