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** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]].
 
** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]].
 
** Lubricated ultrasound transducer is inserted into the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vagina]] and held in the fornix vagina.
 
** Lubricated ultrasound transducer is inserted into the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vagina]] and held in the fornix vagina.
** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized.
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** [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized.
 
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
 
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
 
** Follicular fluid containing the [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocyte]] is aspirated under constant vacuum.
 
** Follicular fluid containing the [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocyte]] is aspirated under constant vacuum.
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