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***One preparation of secondary antibody can be used to test many sera
***One preparation of secondary antibody can be used to test many sera
***By using a mixture of primary antibodies, it is possible to detect the relative expressions of different antigens in the same cell
***By using a mixture of primary antibodies, it is possible to detect the relative expressions of different antigens in the same cell
***Quite often loss of antibody is sustained during the conjugation- in the indirect method the primary antibodies do not need to be conjugated, so this limiting factor is reduced.
==Applications==
*Immunofluorescence is often used to identify populations of cells, and was successfully employed in the identification of the CD4+ and CD8+ subpopulations of T cells
*Identifying bacterial species
*Detecting antigen-antibody complexes in autoimmune diseases
*Detecting complement components in tissues
*Localising hormones, antigens and other cellular products in tissue sampler and subcellular compartments
*Mapping the molecular architecture of tissues in relation to gross anatomy
==Developments==
===Confocal microscopy===