6,502
edits
Changes
Jump to navigation
Jump to search
Bovine Viral Diarrhoea Virus (view source)
Revision as of 19:25, 23 August 2010
, 19:25, 23 August 2010→Virus Structure
===Virus Structure===
===Virus Structure===
Donis, 1995 Dubovi, 1990
Donis, 1995 Dubovi, 1990
The BVDV genome comprises a single strand of positive sense RNA which is around 12.3 kilobases in length<sup>3</sup>. The genome is read in one 3898-codon open reading frame, commencing at nucleotide 386, that contains no non-coding sequences. BVDV polyprotein is translated directly from the ORF and is cleaved by viral and cellular proteinases to form mature viral proteins<sup>3, 4</sup>.
The BVDV genome comprises a single strand of positive sense RNA which is around 12.3 kilobases in length<sup>3</sup>. The genome is read in one 3898-codon open reading frame that contains no non-coding sequences. BVDV polyprotein is translated directly from the ORF and is cleaved by viral and cellular proteinases to form mature viral proteins<sup>3, 4</sup>.
The 5’ and 3’ untranslated regions (UTRs) are located at either end of the ORF. Unlike eukaryotic mRNA, BVDV genomic RNA has neither the 5’ methylated cap nor the 3’ poly-A tail at these positions. Instead, the UTRs are significantly long, at approximately 385 and 226 nucleotides for the 5’ and 3’ UTRs respectively (Donis, 1995). This allows them to accommodate a variety of functions normally conferred by the 5’ cap and the poly-A region, including the control of translation initiation and RNA stability. Entry of replicases required to produce viral progeny from the RNA template is also guided by the UTRs.
The 5’ and 3’ untranslated regions (UTRs) are located at either end of the ORF. Unlike eukaryotic mRNA, BVDV genomic RNA has neither the 5’ methylated cap nor the 3’ poly-A tail at these positions. Instead, the UTRs are significantly long, at approximately 385 and 226 nucleotides for the 5’ and 3’ UTRs respectively (Donis, 1995). This allows them to accommodate a variety of functions normally conferred by the 5’ cap and the poly-A region, including the control of translation initiation and RNA stability. Entry of replicases required to produce viral progeny from the RNA template is also guided by the UTRs.