BVDV is diagnosed by the detection of virus or antibody in blood and milk samples. Antibody detection shows exposure of the herd to disease, whereas tests for antigen identify PI animals which may be antibody negative (Brownlie et. al, 2000).
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There are several techniques available for the laboratory diagnosis of BVD.
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Laboratory techniques for diagnosis of
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BVD virus
Detection of antibody
Detection of antibody
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Serum neutralisation
Serum neutralisation depends on the ability of antibodies in
Serum neutralisation depends on the ability of antibodies in
the serum to neutralise BVD virus and thereby prevent
the serum to neutralise BVD virus and thereby prevent
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days to obtain a result but is dependent on cell culture
days to obtain a result but is dependent on cell culture
facilities and an experienced observer.
facilities and an experienced observer.
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ELISA
ELISA
An enzyme-linked immunosorbent assay (ELISA) technique
An enzyme-linked immunosorbent assay (ELISA) technique
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takes one day. It requires purified ingredients but is simple to
takes one day. It requires purified ingredients but is simple to
operate and the results can, if necessary, be recorded by eye.
operate and the results can, if necessary, be recorded by eye.