Difference between revisions of "Collecting Pathological Samples"

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#REDIRECT[[:Category:Pathological Sample Collection]]
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==Blood samples==
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{|border="2" width="800px" align="center" cellspacing="0" cellpadding="4" rules="all" style="margin:1em 1em 1em 0; border:solid 1px #AAAAAA; border-collapse:collapse;empty-cells:show"
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!bgcolor="#A7C1F2" width="180px"|Test Required
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!bgcolor="#A7C1F2"|Sample Required
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!bgcolor="#A7C1F2"|Comments
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|-
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!align="left" bgcolor="#F2F2F2"|Haematology
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|bgcolor="#F2F2F2"|EDTA
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|bgcolor="#F2F2F2"|Mix gently immediately after collection
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|-
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!align="left"|Biochemistry
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|Serum
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|Centrifuge after permitting sufficient clotting time (min 2 hours)
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|-
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!align="left"|Fibrinogen
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|Citrate
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|Mix gently immediately after collection
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|-
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!align="left" bgcolor="#F2F2F2"|SAA
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|bgcolor="#F2F2F2"|Serum
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|bgcolor="#F2F2F2"|Needs to be processed quickly as the half life is very short
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|-
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!align="left" bgcolor="#F2F2F2"|Electrolytes
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|bgcolor="#F2F2F2"|Heparin
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|bgcolor="#F2F2F2"|Mix gently immediately after collection
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|-
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!align="left"|Glucose
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|Fluoride oxalate
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|Can use fresh whole blood if tested immediately
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|-
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!align="left" bgcolor="#F2F2F2"|Insulin
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|bgcolor="#F2F2F2"|Serum
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|bgcolor="#F2F2F2"|Fasting sample required
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|-
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!align="left"|Prothrombin clotting time
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|Citrate
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|A normal sample is required for comparison
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|-
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!align="left"|Progesterone
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|Serum
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|
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|-
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!align="left"|Oestrone Sulphate
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|Serum
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|
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|-
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!align="left" bgcolor="#F2F2F2"|Testosterone
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|bgcolor="#F2F2F2"|Serum
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|bgcolor="#F2F2F2"|Request Testosterone before Inhibin for GCTs
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|-
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!align="left" bgcolor="#F2F2F2"|IgG
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|bgcolor="#F2F2F2"|Serum
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|bgcolor="#F2F2F2"|Measurement techniques vary so check sample requirements
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|-
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!align="left" bgcolor="#F2F2F2"|ACTH
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|bgcolor="#F2F2F2"|EDTA plasma
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|bgcolor="#F2F2F2"| Freeze plasma as soon after collection as poss (max 2 hours)
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|-
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!align="left" bgcolor="#F2F2F2"|Selenium
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|bgcolor="#F2F2F2"|Heparin/Serum
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|bgcolor="#F2F2F2"|Send Heparin for deficiency testing, serum for toxicity testing.
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|-
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!align="left"|Zinc
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|Serum
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|Avoid using tubes with a rubber bung
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|-
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!align="left" bgcolor="#F2F2F2"|Drugs testing
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|bgcolor="#F2F2F2"|Serum or Heparin
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|bgcolor="#F2F2F2"|Send to HFL
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|-
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!align="left"|Virus Titres e.g. EHV
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|Serum
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|Take 2 samples 14 days apart (freeze 1st)
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|-
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!align="left"|Tapeworm ELISA
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|Serum
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|
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|-
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|}
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==Bacteriology Samples==
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==Skin Scrapes==
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==Urine Samples==
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==Peritoneal and Joint taps==
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==Tracheal Washes and BALs==
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==WECs==

Revision as of 20:58, 21 September 2010

Blood samples

Test Required Sample Required Comments
Haematology EDTA Mix gently immediately after collection
Biochemistry Serum Centrifuge after permitting sufficient clotting time (min 2 hours)
Fibrinogen Citrate Mix gently immediately after collection
SAA Serum Needs to be processed quickly as the half life is very short
Electrolytes Heparin Mix gently immediately after collection
Glucose Fluoride oxalate Can use fresh whole blood if tested immediately
Insulin Serum Fasting sample required
Prothrombin clotting time Citrate A normal sample is required for comparison
Progesterone Serum
Oestrone Sulphate Serum
Testosterone Serum Request Testosterone before Inhibin for GCTs
IgG Serum Measurement techniques vary so check sample requirements
ACTH EDTA plasma Freeze plasma as soon after collection as poss (max 2 hours)
Selenium Heparin/Serum Send Heparin for deficiency testing, serum for toxicity testing.
Zinc Serum Avoid using tubes with a rubber bung
Drugs testing Serum or Heparin Send to HFL
Virus Titres e.g. EHV Serum Take 2 samples 14 days apart (freeze 1st)
Tapeworm ELISA Serum

Bacteriology Samples

Skin Scrapes

Urine Samples

Peritoneal and Joint taps

Tracheal Washes and BALs

WECs