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| + | #REDIRECT[[:Category:Piroplasmida]] |
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| − | '''[[Babesia|''Babesia'']]
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| − | ==''Cytauxzoon felis''==
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| − | *Cytauxzoon is classified in the order ''Piroplasmida'' and family ''Theileriidae''
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| − | **This family has both an erythrocytic and a tissue (leukocytic) phase
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| − | *The ''Babesiidae'', a related family, is characterized by having a primarily erythrocytic phase in the mammalian host
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| − | **Its morphological features are indistinguishable from the erythrocytic form of Cytauxzoon
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| − | *''Cytauxzoon felis'', ''B. equi'', and ''B. rodhaini'' have been linked to both the babesias and theilerias by RNA gene sequence analysis
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| − | **It has been suggested that these organisms be reclassified within a separate family
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| − | '''Life Cycle'''
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| − | *Large schizonts of ''C. felis'' develop in [[Macrophages - WikiBlood|macrophages]]
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| − | **In Theileria the exoerythrocytic stage occurs primarily within [[Lymphocytes - WikiBlood|lymphocytes]]
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| − | *In ''C. felis'', schizonts develop within mononuclear phagocytes, initially as indistinct vesicular structures and later as large, distinct nucleated schizonts that actively undergo division by true schizogony and binary fission
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| − | *Later in the course of the disease, schizonts develop buds (merozoites) that separate and eventually fill the entire host cell
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| − | *Each schizont may contain numerous merozoites
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| − | **Ultrastructurally, schizonts lack a parasitophorous vacuole, and individual merozoites possess rhoptries
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| − | *The host cell ruptures, releasing merozoites into the tissue fluid and blood
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| − | *Merozoites are then believed to enter erythrocytes to form the intraerythrocytic stage
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| − | *Merozoites appear in [[Macrophages - WikiBlood|macrophages]] one to three days before they are observed in [[Erythrocytes - WikiBlood|erythrocytes]]
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| − | '''Pathogenicity'''
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| − | *[[Ticks|Ticks]] are implicated as the natural vector for ''Cytauxzoon''
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| − | **Most cases of infection have been associated with the presence of these parasites on the hosts
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| − | **Experimentally, ''Dermacentor variabilis'' can transmit the organism from bobcats to domestic cats. In a white tiger that developed a natural, fatal infection in Florida, two female Lone Star ticks (''Amblyomma americanum'') were present on the inguinal skin.
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| − | *Clinically, the disease in cats is characterized by fever, depression, dyspnoea, anorexia, lymphadenopathy, anaemia and icterus leading to death in three to six days
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| − | *Gross findings include pale or icteric mucous membranes, petechiae and ecchymoses in the [[Lungs - Anatomy & Physiology|lung]], [[Heart - Anatomy & Physiology|heart]], [[Lymph Nodes - Anatomy & Physiology|lymph nodes]] and on mucous membranes, splenomegaly, lymphadenomegaly, and hydropericardium
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| − | *Microscopically, numerous large schizonts are present within the cytoplasm of endothelial-associated [[Macrophages - WikiBlood|macrophages]]
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| − | **Infected macrophages become markedly enlarged (up to 75μm) and may occlude the lumen of numerous vessels of many tissues, in particular the [[Lungs - Anatomy & Physiology|lungs]]
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| − | **Minimal inflammatory reaction is present in tissues
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| − | '''Diagnosis'''
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| − | *Merozoites within [[Erythrocytes - WikiBlood|erythrocytes]], best seen on peripheral blood or tissue impressions, are variable in morphology and can occur as round, oval, or signet ring-shaped bodies
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| − | **Are 1-5 micrometers in diameter
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| − | **Small, peripherally placed basophilic nucleus
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| − | *Organisms that must be distinguished from the intraerythrocytic phase of ''C. felis'' include ''Babesia'' and ''Hemobartonella''
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| − | **The blood stage may appear similar to the ring forms of ''Hemobartonella'' and to the piriforms of ''Babesia''
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| − | **Unlike ''Cytauxzoon'', ''babesiosis'' and ''hemobartonellosis'' do not have a tissue stage of infection
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| − | *Differential diagnosis for the tissue phase of ''cytauxzoonosis'' includes other small (less than 5 μm), intrahistiocytic organisms such as ''Toxoplasma'', ''Leishmania'' and ''Histoplasma''
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| − | ==''Theileria''==
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| − | [[Image:Theileria parva life cycle.jpg|thumb|right|150px|''Theileria parva'' Life Cycle Diagram - Dennis Jacobs & Mark Fox RVC]]
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| − | [[Image:Lymph node smear East Coast Fever.jpg|thumb|right|150px|Lymph node smear of a cow with East Coast Fever - Drs. Elizabeth Howerth and Bruce LeRoy, Department of Pathology, UGA College of Veterinary Medicine]]
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| − | [[Image:H and E stain brain East Coast Fever.jpg|thumb|right|150px|H and E stain of brain and meningal vessels of a cow with East Coast Fever - Drs. Elizabeth Howerth and Bruce LeRoy, Department of Pathology, UGA College of Veterinary Medicine]]
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| − | [[Image:Theileria cervi.jpg|thumb|right|150px|''Theileria cervi'' (deer) - Drs. Elizabeth Howerth and Bruce LeRoy, Department of Pathology, UGA College of Veterinary Medicine]] | |
| − | *Main species of veterinary importance is ''Theileria parva''
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| − | **Causes '''East Coast Fever'''
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| − | ***Severe, proliferative lymphatic disease of cattle
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| − | ***Central and Eastern Africa
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| − | ***Transmitted by [[Hard Ticks - Overseas|''Rhipicephalus appendiculatus'']]
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| − | ***[[Ticks#Disease Transmission|Trans-stadial]] transmission
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| − | *Other ''Theileria'' species cause production losses in cattle and sheep in the Middle East, Mediterranean and in Northern Africa
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| − | '''Life Cycle'''
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| − | *Incubation phase lasts 1 week
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| − | *Lymphoblast proliferation
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| − | **Local [[Lymph Nodes - Anatomy & Physiology|lymph node]] first infected then spreads through body
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| − | **Occurs in week two
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| − | *Lymphoid depletion
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| − | **[[Lymphocytes - WikiBlood|Lymphocytes]] killed
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| − | **Decreases lymphopoiesis
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| − | **Occurs in week 3
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| − | *Total incubation period takes about 18 days
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| − | '''Diagnosis'''
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| − | *Clinical signs
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| − | **Pyrexia
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| − | **Enlarged local [[Lymph Nodes - Anatomy & Physiology|lymph node]]
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| − | ***Usually parotid [[Lymph Nodes - Anatomy & Physiology|lymph node]] as [[Hard Ticks - Overseas|''Rhipicephalus appendiculatus'']] feeds in the ear
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| − | **Loss of condition
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| − | *Examine Giemsa stained smears of:
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| − | **Local [[Lymph Nodes - Anatomy & Physiology|lymph node]] aspirated for schizonts
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| − | **Blood smears for piroplasms in red blood cells
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| − | *Post-mortem
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| − | **Pulmonary oedema
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| − | **Gut mucosal haemorrhages
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| − | **[[Lymph Nodes - Anatomy & Physiology|Lymph node]] and [[Spleen - Anatomy & Physiology|splenic]] cellular atrophy
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| − | '''Control'''
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| − | *Integrated control of both the [[Tick Control|tick vector]] and disease
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| − | **[[Vaccines - WikiBlood|Vaccination]] and [[Ectoparasiticides]]
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| − | *Current [[Vaccines - WikiBlood|vaccination]] is live unattentuated
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| − | **Contains frozen stabilate of ground up tick gut containing infective sporozoites
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| − | **Long lasting oxytetracycline administered at the same time to slow down schizogony giving the immune response time to develop
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| − | ==[[Protozoa Flashcards - Wikibugs#Piroplasmida|Piroplasmida Flashcards]]==
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