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| | <categorytree mode=pages>Staphylococcus species</categorytree> | | <categorytree mode=pages>Staphylococcus species</categorytree> |
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| − | [[Staphylococcus species - Introduction]]
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| − | ===Overview===
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| − | There are a large number of ''Staphyloccocus spp'' present in the environment and many are commensals of the skin and mucous membranes of animals and man. Many are opportunistic pathogens causing pyogenic infections as a result of trauma, immunosuppression and secondary to other infections.
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| − | *Enodgenous or exogenous infections
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| − | *'''Coagulase-positive''' ''S. aureus'' and ''S. intermedius'' as well as ''S. hyicus'' are important pathogens of animals
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| − | *Fairly stable in environment
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| − | *Strains selective for particular species
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| − | *Cause mastitis, tick pyaemia, exudative epidermitis, botryomycosis and pyoderma
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| − | ===Characteristics===
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| − | Although individual species may vary slightly, most have similar characteristics.
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| − | *Gram-positive
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| − | *Form grape-like clusters
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| − | *Moderate sized, white/golden colonies
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| − | *Facultative anaerobes
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| − | *Grow on non-enriched media
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| − | *Non motile
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| − | *Catalase-positive
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| − | *Haemolytic, 4 haemolysins recognised
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| − | *Oxidase-negative
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| − |
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| − | ===Detection & Differentiation===
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| − | The most simple method of detection is to gram stain a sample, usually puss, and to then visualise the distinctive clusters under a microscope. This provides a basic identification of the type of bacteria, however occasionally it is necessary to identify the species that causing the infection. This can be done using various techniques based on the different characteristics of the species. Selective agar such as blood, for haemolysin producing species such as ''S. aureus'' and ''S. intermedius'' as well as non haemolytic species such as ''S. hyicus''. MacConkey agar can be used to identify. PCR is the most specific method of determining the species of bacteria but requires laboratory analysis of samples and is only usually used in research.
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| − | *Coagulase-negative strains vary in ability to cause haemolysis
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| − | *Slide test to detect bound coagulase (clumping factor) on surface of bacteria; bacteria clump within 1-2 minutes
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| − | *Tube test detects free coagulase, secreted by bactera; positive result indicated by clot formation in tube following 24-hour incubation; free coagulase converts prothrombin to thrombin which converts fibrinogen to fibrin
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| − | ===Pathogenesis and pathogenicity===
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| − | *Cause suppurative lesions
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| − | *Trauma or immunosuppression predispose to infection
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| − | *Capsular polysaccharide, teichoic acids and potein A prevent opsonisation and therefore phagocytosis
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| − | *Cell wall proteins bind fibronectin and fibrinogen, allowing bacteria to attched to damaged tissues
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| − | *Coagulase, DNase and protein A production mark pathogenicity
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| − | *Haemolysins made by ''S. aureus'' and ''S. intermedius'' act as toxins
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| − | *Alpha-haemolysin prouces a narrow zone of complete haemolysis; this toxin causes necrosis and targets mast cells and plasma cells, whose contents cause damage
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| − | *Beta-haemolysin produces a wide zone of incomplete haemolysis; damages membranes and causes leakage, contributing to necrosis
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| − | *Also gamma and delta toxins
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| − | *Toxic shock syndrome toxin acts as a superantigen, which causes T cell proliferation and production of cytokines, leading to cardiovascular shock, with microthrombus formation in capillaries
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| − | *Enterotoxins produced by some strains of ''S. aureus'' cause food poisoning in humans
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| − | *Proteases, hyaluronidases and lipases facilitate survival of bacteria and spread and tissue destruction
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| − | ===Diagnosis===
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| − |
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| − | *Clusters of bacteria in Gram-stained smears of pus
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| − | *Culture on selective blood agar and MacConkey agar
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| − | *No growth on MacConkey
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| − | *Colony characteristics, haemolysis, catalase and coagulase production
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| − | *Phage typing for epidemiological studies
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| − | ===Infections in dogs and cats===
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| − | *''S. intermedius'' causes [[Bacterial skin infections - Pathology#Deep pyoderma|deep pyoderma]], otitis externa, mastitis, endometritis, cystitis, osteomyelitis, wound infections, [[Joints Inflammatory - Pathology#In Dogs|dyscospondylitis]]
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| − | *''S. aureus'' may cause gastroenteritis
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| − |
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| − | ===Coagulase-negative staphylococci===
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| − |
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| − | *Usually harmless commensals or secondary invaders
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| − | *May adhere to indwelling catheters leading to urinary tract infections
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| − | *Often display multiple antibiotic resistance
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| − | ==Enteritis==
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| − | * 30% strains of ''[[Staphylococcus aureus]]'' produce potent enterotoxin. T
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| − | ** Protein and heat-stable
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| − | ** Responsible for staphylococcal food poisoning in man.
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| − | * Every reason to assume that acute gastro-intestinal disturbance in small animals may be caused by these enterotoxins BUT not well documented.
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| − | * Symptoms last 24-36 hours and include:
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| − | ** Acute vomiting
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| − | ** Diarrhoea
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| − | ** Pain
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| − | * The enterotoxins are superantigens.
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| − | ** Induce release of cytokines from lymphocytes
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| − | '''sheep'''
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| − |
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| − | *[[:Category:Staphylococcus species|'''''Staphylococci''''']]
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| − | **Sporadically infect joints
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| − | **May complicate 'tick-borne fever'
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| − | '''pigs'''
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| − | **Cause purulent arthritis
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| − | **In one or more joints
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| − | **Commonly contaminate wounds
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| − | '''dogs'''
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| − | **May cause '''discospondylitis'''
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| − | **Destructive inflammatory lesion
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| − | **Bacterial localise in ''[[Joints - Anatomy & Physiology#Fibrocartilagenous joints|''annulus fibrosis'']] -> may spread to local bone
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| | [[Category:Bacterial Organisms]] | | [[Category:Bacterial Organisms]] |
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| | [[Category:Cocci]] | | [[Category:Cocci]] |
| | [[Category:Enteritis,_Bacterial]] | | [[Category:Enteritis,_Bacterial]] |
| − | [[Category:To_Do_-_NickJ]]
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