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In Vitro Fertilization - Anatomy & Physiology (view source)
Revision as of 07:33, 17 July 2008
, 07:33, 17 July 2008→Oocyte Collection
* Donor is superovulated to provide large numbers of mature follicles.
* Donor is superovulated to provide large numbers of mature follicles.
* Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
* Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
* Oocytes captured in vitro.
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
** Follicular fluid containing the oocyte is aspirated under constant vacuum.
** Follicular fluid containing the oocyte is aspirated under constant vacuum.
* Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
** Could also be cultured in a 'nurse animal' in vivo.
* Embryos are incubated for a further period to the appropriate stage for transfer.
* Embryos transferred to recipient female.
* Can be transferred at the correct stage of the recipient's oestrous cycle.
* Embryos transferred around the blastocyst stage.
* Success rates are improving.
* Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
* Increases incidence of large offspring syndrome.