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→Diagnosis
*Should soon be replaced by immunochromatography-bedside ELISA to allow on-farm diagnosis
*Should soon be replaced by immunochromatography-bedside ELISA to allow on-farm diagnosis
*'''Virus isolation''' can also be performed in kidney culture cells, and then serotyped by ELISA
*'''Virus isolation''' can also be performed in kidney culture cells, and then serotyped by ELISA
*Serology for virus '''antibody''' can determine past infection
**ELISAs used to detect subclinical carrier sheep
**Cannot be done on vaccinated animals
*RT-PCR has been suggested for on-farm diagnosis, but has flaws:
**RNA is readily degraded by tissue enzymes
**RNA must be purified before converting to DNA for PCR
**False positives can occur easily by contamination with previously amplified DNA
====Control====
====Control====