Erythrocytes

Erythrocytes

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Also known as red blood cells (RBCs)

Erythrocytes deliver oxygen to, and remove carbon dioxide from tissues.

Development

Erythrocytes are derived from the stem cell (CFU-GEMM) and formed in a process known as erythropoiesis.

Erythrocyte Structure

Avian Erythrocytes

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Mammals

Erythrocytes are small (4-8µm), circular, anucleate biconcave cells that lack organelles.

The cell's membrane is a lipid bilayer which contains two different protein groups. Integral membrane proteins, which make up most of the proteins, and peripheral membrane proteins. The arrangement of these proteins makes the membrane elastic but stable and contributes towards the biconcave shape.

The biconcave shape increases the cell's surface area by 20-30%, thereby increasing the probability of oxygen binding with the haemoglobin molecule within the RBC.

Birds

In birds erythrocytes are ovoid and contain a nucleus.

Function

RBCs contain haemoglobin, an oxygen binding protein that is responsible for the transport of oxygen from the lungs to tissues, and the transportation of carbon dioxide from the tissues to the lungs for elimination.

Blue(α) & orange(β) are globin chains

Red objects represent haem groups

Haemoglobin (Hb) is a metalloprotein that binds oxygen reversibly and accounts for almost all of the protein in the erythrocyte.

When bound to oxygen it is referred to as oxyhaemoglobin and when unbound, deoxyhaemoglobin. Haem gives blood its colour, and this colour depends on its saturation. When fully saturated with four oxygen molecules it appears red and when completely unsaturated it appears blue-red.

Haemoglobin also acts as an extracellular buffer

• Hb(O₂)H + H₂ ↔ Hb(O₂)^- + H₃O⁺
  • pK_a = 6.6
• HbH + H₂O ↔ Hb^- + H₃O⁺
  • pK_a = 7.8

Haemoglobin Structure

Haemoglobin is composed of globin and four haem groups which contain an iron atom in each haem group. Four oxygen molecules can bind to each haemoglobin - one to each heam group via the iron when in its ferrous state.

Globin

Globin is composed of two polypeptide groups each composed of two polpeptides chains. The chains are identical within the group, but differ between the groups. Globin varies between species and individuals within a species due to changes in the polypeptide chain sequence.

Haem

Each of the haem groups has an iron atom at its centre and each iron atom binds one molecule of oxygen. Haem groups are identical in all species.

Types

There are three types of haemoglobin: embryonic, foetal and adult. Embryonic is present in the early stages of foetal development. Foetal haemoglobin has a higher affinity for oxygen than adult haemoglobin and is replaced by adult haemoglobin shortly after birth. Dogs do not have foetal or embryonic haemoglobin. Horses do not have embryonic haemoglobin and their foetal haemoglobin is structurally identical to adult haemoglobin and as such some would say that there is no foetal haemoglobin. Pig (and horse) foetal haemoglobin has the same affinity for oxygen as their adult haemoglobin.

Chemistry

• One gram of haemoglobin binds 1.34g of oxygen.
• Haemoglobin concentration (g/l) varies between species
  • Poultry: 90
  • Ruminants: 100-120
  • Dogs: 150

Antigens

The RBC surface contains glycoproteins that are specific to the individual and potentially antigenic. Plasma contains antibody like substances known as agglutins - mixing RBCs with the blood of another individual can create an agglutination reaction where the plasma agglutins and the RBC glycoproteins bind together. This is the basis of a transfusion reaction and other blood type abnormalities associated with reproduction such as neonatal isoerythrolysis in horses and fading kitten syndrome.

Degradation

Erythrocytes have a limited life span and as they age their membranes become more fragile leading to a loss of function and swelling. Damage occurs as the fragile cells pass through narrow capillaries which occurs particularly in the spleen. Ageing also causes sialic acid to be lost from the membrane and this exposes galactose moieties leading to phagocytosis.

Life Span

The life span of the erythrocyte varies from 70-160 days in domestic species although the life span is often shorter in juvenile animals (e.g. calves and lambs) compared to adult animals. Erythrocytes in small animals have a shorter life span than in the larger domestic species.

Life span (days)
Cat	Pig	Dog	Goat	Horse	Sheep	Cattle
70	85	120	125	145	150	160

Transfused dog erythrocytes last around 21 days

Breakdown

Around 90% of erythrocytes are phagocytised by macrophages in the liver, spleen and bone marrow, with the other 10% breaking down in circulation.

Following phagocytosis, haemoglobin is broken down into haem and globin. Globin, a protein, is hydrolysed into amino acids while the haem is transformed into biliverdin and then into bilirubin before being excreted into the blood stream bound to albumin. In the liver it is conjugated and excreted into bile which in turn is excreted into the gastro-intestinal system. The iron from haem is either stored as insoluble ferritin in the liver (in hepatocytes and macrophages) or it is directly transported to the bone marrow for reuse in erythropoiesis.

Haem breakdown pathway

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Erythrocytes Learning Resources
PowerPoint Selection of relevant PowerPoint tutorials	Tutorial about histology of blood cells