Difference between revisions of "Bacillus species"

From WikiVet English
Jump to navigation Jump to search
Line 29: Line 29:
 
*Motile
 
*Motile
 
*Catalase positive, oxidase negative
 
*Catalase positive, oxidase negative
*''Bacillus anthracis'' colonies are up to 5mm diameter, flat, dry, grey, with a ground-glass appearance; curled outgrowthsfrom the edge of the colony give a 'medusa head' appearance; non-haemolytic (differentiate from ''Bacillus cereus'')
+
*''Bacillus anthracis'' colonies are up to 5mm diameter, flat, dry, grey, with a ground-glass appearance; curled outgrowth sfrom the edge of the colony give a 'medusa head' appearance; non-haemolytic (differentiate from ''Bacillus cereus'')
 
*''Bacillus licheniformis'' forms dull, rough, wrinkled colonies, with hair-like outgrowths
 
*''Bacillus licheniformis'' forms dull, rough, wrinkled colonies, with hair-like outgrowths
 
*Biochemical tests for identification
 
*Biochemical tests for identification
Line 53: Line 53:
 
**Protective antigen binds to the cell receptor to allow action of the other factors
 
**Protective antigen binds to the cell receptor to allow action of the other factors
 
**The toxin kills phagocytes, increases capillary permeability and interferes with clotting cascade
 
**The toxin kills phagocytes, increases capillary permeability and interferes with clotting cascade
 +
**Capillary thrombosis; leakage of fluid through damaged capillary endothelium
 +
**Systemic shock from circulatory collapse, haemorrhage and oedema lead to death of the animal
 
**Causes tissues to darken and swell due to oedema and necrosis
 
**Causes tissues to darken and swell due to oedema and necrosis
 
*Clinical signs:
 
*Clinical signs:
**Cattle:  
+
**Cattle/sheep:  
 
***Fatal peracute septicaemia
 
***Fatal peracute septicaemia
***Capillary thrombosis; leakage of fluid through damaged capillary endothelium
+
***Animals usually found dead
***Systemic shock from circulatory collapse, haemorrhage and oedema lead to death of the animal
+
***Pyrexia, depression, congested mucous membranes and petechiae before death
 +
***Abortion, subcutaneous oedema and dysentry in animals surviving more that one day
 +
***Post mortem: bloat, incomplete rigor mortis, ecchymoses, oedema, dark unclotted blood, blooy fluid in body cavities, splenomegaly
 
**Pigs:
 
**Pigs:
 
***Subacute anthrax with oedematous pharyngeal swelling; intestinal form with high mortality
 
***Subacute anthrax with oedematous pharyngeal swelling; intestinal form with high mortality
 +
 
**Horses:
 
**Horses:
 
***Subacute anthrax with localised oedema; septicaemia with colic and enteritis
 
***Subacute anthrax with localised oedema; septicaemia with colic and enteritis

Revision as of 13:15, 17 May 2008

BACK TO INFECTIOUS AGENTS AND PARASITES
BACK TO BACTERIA



Overview

  • Mostly non-pathogenic environmental organisms
  • Bacillus anthracis causes anthrax
  • Anthrax is a severe disease affecting all mammals worldwide
  • Ruminants are highly susceptible to anthrax, dying of a septicaemic form
  • Pigs and horses are moderately susceptible, but carnivores are fairly resistant
  • Bacillus licheniformis may cause shoradic abortions in cattle and sheep
  • Bacillus cereus causes food poisoning in humans and mastitis in cows


Characteristics

  • Large, Gram positive rods
  • Produce endospores
  • Aerobes or facultative anaerobes
  • Grow on non-enriched media
  • Motile
  • Catalase positive, oxidase negative
  • Bacillus anthracis colonies are up to 5mm diameter, flat, dry, grey, with a ground-glass appearance; curled outgrowth sfrom the edge of the colony give a 'medusa head' appearance; non-haemolytic (differentiate from Bacillus cereus)
  • Bacillus licheniformis forms dull, rough, wrinkled colonies, with hair-like outgrowths
  • Biochemical tests for identification
  • Can often tolerate adverse environmental conditions


Bacillus anthracis

  • Epidemiology:
    • Saprophyte in soil
    • Endospore formation allows persistence and spread
    • Endospores survive decades in the soil
    • Outbreaks in herbivores grazing pastures contaminated by spores from buried carcases
    • Infection usually by ingestion of spores
  • Pathogenesis and pathogenicity:
    • Capsule and toxin (encoded by separate plasmids) required for virulence
    • Capsule composed of homopolymer of D-isomer of glutamic acid allows survival in the body by resisting phagocytosis
    • Capsule stains mauve with polychrome methylene blue in the MacFadyean's reaction - identify anthrax in blood samples
    • Bacilli appear as chains of dark blue, square-ended rods surounded with the capsule
    • Extracellular toxin (holotoxin) composed of oedema factor, protective antigen and lethal factor
    • Oedema factor is an adenylate cyclase which increases intracellular cAMP concetrations, causing fluid accumulation and damaging neutrophils
    • Lethal factor causes release of cytokines from macrophages
    • Protective antigen binds to the cell receptor to allow action of the other factors
    • The toxin kills phagocytes, increases capillary permeability and interferes with clotting cascade
    • Capillary thrombosis; leakage of fluid through damaged capillary endothelium
    • Systemic shock from circulatory collapse, haemorrhage and oedema lead to death of the animal
    • Causes tissues to darken and swell due to oedema and necrosis
  • Clinical signs:
    • Cattle/sheep:
      • Fatal peracute septicaemia
      • Animals usually found dead
      • Pyrexia, depression, congested mucous membranes and petechiae before death
      • Abortion, subcutaneous oedema and dysentry in animals surviving more that one day
      • Post mortem: bloat, incomplete rigor mortis, ecchymoses, oedema, dark unclotted blood, blooy fluid in body cavities, splenomegaly
    • Pigs:
      • Subacute anthrax with oedematous pharyngeal swelling; intestinal form with high mortality
    • Horses:
      • Subacute anthrax with localised oedema; septicaemia with colic and enteritis


Bacillus licheniformis

  • Widespread in the environment
  • Associated with food spoilage
  • Abortion in cattle and sheep, possibly from spoiled silage or hay


Bacillus cereus

  • Mastitis in cattle
  • Food poisoning and eye infections in humans