Difference between revisions of "Semen Sexing - Anatomy & Physiology"
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<big><center>[[Reproductive_System#Reproductive_Technologies|'''BACK TO REPRODUCTIVE TECHNOLOGIES''']]</center></big> | <big><center>[[Reproductive_System#Reproductive_Technologies|'''BACK TO REPRODUCTIVE TECHNOLOGIES''']]</center></big> | ||
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| + | == Principle == | ||
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| + | * X and Y chromosomes contain different quantities of DNA. | ||
| + | * X-bearing sperm contain 2.8-4.2% more DNA (species dependent) than Y-bearing sperm. | ||
| + | * This allows them to be sorted into two subpopulations. | ||
| + | * Separation requires the uptake of DNA stain/dye called Fluorochrome into living and dead sperm. | ||
| + | * X-bearing sperm take up more dye than Y-bearing sperm. | ||
| + | * Vital dyes emit light at a specific wavelength when excited or activated. | ||
| + | ** X-bearing sperm will emit a larger signal than Y-bearing sperm. | ||
| + | * This allows the sperm to be sorted by '''flow cytometry'''. | ||
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| + | ---- | ||
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| + | == Flow Cytometry == | ||
Revision as of 10:14, 17 July 2008
Principle
- X and Y chromosomes contain different quantities of DNA.
- X-bearing sperm contain 2.8-4.2% more DNA (species dependent) than Y-bearing sperm.
- This allows them to be sorted into two subpopulations.
- Separation requires the uptake of DNA stain/dye called Fluorochrome into living and dead sperm.
- X-bearing sperm take up more dye than Y-bearing sperm.
- Vital dyes emit light at a specific wavelength when excited or activated.
- X-bearing sperm will emit a larger signal than Y-bearing sperm.
- This allows the sperm to be sorted by flow cytometry.