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In Vitro Fertilization - Anatomy & Physiology (view source)
Revision as of 09:45, 17 July 2008
, 09:45, 17 July 2008→Oocyte Collection
* Donor is superovulated to provide large numbers of mature follicles.
* Donor is superovulated to provide large numbers of mature follicles.
* Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
* Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
* Oocytes captured in vitro.
* [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|Oocytes]] captured in vitro.
** Follicular fluid is aspirated.
** Follicular fluid is aspirated.
** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]].
** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]].
** Repeated 2-3 times to dislodge oocytes.
** Repeated 2-3 times to dislodge [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocytes]].
** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized.
** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized.
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle.
** Follicular fluid containing the oocyte is aspirated under constant vacuum.
** Follicular fluid containing the [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocyte]] is aspirated under constant vacuum.
* Aspirated oocytes are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_-_Anatomy_%26_Physiology#Capacitation|capacitated]] spermatozoa.
* Aspirated [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_-_Anatomy_%26_Physiology#Capacitation|capacitated]] spermatozoa.
** Could also be cultured in a 'nurse animal' in vivo.
** Could also be cultured in a 'nurse animal' in vivo.
* Embryos are incubated for a further period to the appropriate stage for transfer.
* Embryos are incubated for a further period to the appropriate stage for transfer.