Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
Jump to navigation
Jump to search
Line 30: | Line 30: | ||
* Embryos are incubated for a further period to the appropriate stage for transfer. | * Embryos are incubated for a further period to the appropriate stage for transfer. | ||
* Embryos transferred to recipient female. | * Embryos transferred to recipient female. | ||
− | * Can be transferred at the correct stage of the recipient's oestrous cycle. | + | * Can be transferred at the correct stage of the recipient's [[Female_Reproductive_Tract_-Oestrous_Cycle_-_Anatomy_%26_Physiology|oestrous cycle]]. |
* Embryos transferred around the [[Fertilisation%2C_Implantation_and_Early_Embryonic_Development_-Embryonic_Development_of_the_Blastocyst-_Anatomy_%26_Physiology|blastocyst]] stage. | * Embryos transferred around the [[Fertilisation%2C_Implantation_and_Early_Embryonic_Development_-Embryonic_Development_of_the_Blastocyst-_Anatomy_%26_Physiology|blastocyst]] stage. | ||
* Success rates are improving. | * Success rates are improving. | ||
* Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations. | * Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations. | ||
* Increases incidence of [[Parturition_-_Risks_in_Parturition_-_Anatomy_%26_Physiology#Large_Offspring|large offspring syndrome]]. | * Increases incidence of [[Parturition_-_Risks_in_Parturition_-_Anatomy_%26_Physiology#Large_Offspring|large offspring syndrome]]. |
Revision as of 09:46, 17 July 2008
Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
- Oocytes captured in vitro.
- Direct follicle aspiration:
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.
- Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
- Could also be cultured in a 'nurse animal' in vivo.
- Embryos are incubated for a further period to the appropriate stage for transfer.
- Embryos transferred to recipient female.
- Can be transferred at the correct stage of the recipient's oestrous cycle.
- Embryos transferred around the blastocyst stage.
- Success rates are improving.
- Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
- Increases incidence of large offspring syndrome.