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8 bytes added ,  16:32, 12 June 2010
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Text replace - 'IgG' to 'IgG'
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*There are various methods to separate the antigen-antibody complexes from the free antigen:
 
*There are various methods to separate the antigen-antibody complexes from the free antigen:
 
**Precipitate complexes using secondary isotype-specific anti-immunoglobulin
 
**Precipitate complexes using secondary isotype-specific anti-immunoglobulin
**If complex contains IgG, it can be removed by mixing with formalin-killed ''Staphylococcus aureus''- protein A of ''S. aureus'' has a high affinity for IgG
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**If complex contains [[IgG]], it can be removed by mixing with formalin-killed ''Staphylococcus aureus''- protein A of ''S. aureus'' has a high affinity for [[IgG]]
 
*** Removal of the complex by either of these methods leaves an amount of free labeled antigen in the supernatant (liquid section from precipitation)- the radioactivity of this can be measured and the value taken away from the total amount of labeled antigen added (known amount)= amount of bound labeled antigen
 
*** Removal of the complex by either of these methods leaves an amount of free labeled antigen in the supernatant (liquid section from precipitation)- the radioactivity of this can be measured and the value taken away from the total amount of labeled antigen added (known amount)= amount of bound labeled antigen
 
**A number of solid-phase RIAs have been developed
 
**A number of solid-phase RIAs have been developed

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