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==Introduction==
==Introduction==
==Fluorescent dyes==
==Fluorescent dyes==
If a molecule has the property of fluorescence, it can absorb light of one wavelength (excitation) and emit light of another (emission). Antibodies tagged with these dyes (known as '''fluorochromes''') form immune complexes with specific antigens which can then be indirectly visualised when excited by light of the appropriate wavelength.
If a molecule has the property of fluorescence, it can absorb light of one wavelength (excitation) and emit light of another (emission). Antibodies tagged with these dyes (known as '''fluorochromes''') form immune complexes with specific antigens which can then be indirectly visualised when excited by light of the appropriate wavelength.
*This detector is capable of measuring the strength of emission, so it is possible to sort cells that are negative, slightly positive and highly positive for a specific antigen
*This detector is capable of measuring the strength of emission, so it is possible to sort cells that are negative, slightly positive and highly positive for a specific antigen
*Flow cytometry provides a rapid quantitative technique for antigen detection
*Flow cytometry provides a rapid quantitative technique for antigen detection
[[Category:Immunological Testing]]