Changes

* Donor is superovulated to provide large numbers of mature [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]].

* Donor is superovulated to provide large numbers of mature [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]].

* Pre-ovulatory [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content.

* Pre-ovulatory [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicles]] are collected by a surgical procedure involving aspiration of the follicular content.

* [[The_Ovary_-_Oocytes_- Anatomy & Physiology|Oocytes]] captured in vitro.

* [[Oogenesis - Anatomy & Physiology|Oocytes]] captured in vitro.

** Follicular fluid is aspirated.

** Follicular fluid is aspirated.

** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]].

** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]].

** Repeated 2-3 times to dislodge [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocytes]].

** Repeated 2-3 times to dislodge [[Oogenesis - Anatomy & Physiology|oocytes]].

** [[Ovary - Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] can be visualized.

** [[Ovary - Anatomy & Physiology|Ovary]] transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_- Anatomy & Physiology|follicle]] can be visualized.

** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle.

** Hypodermic needle is advanced through the [[Vagina and Vestibule - Anatomy & Physiology|vaginal wall]] into the antral follicle.

** Follicular fluid containing the [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocyte]] is aspirated under constant vacuum.

** Follicular fluid containing the [[Oogenesis - Anatomy & Physiology|oocyte]] is aspirated under constant vacuum.

* Aspirated [[The_Ovary_-_Oocytes_- Anatomy & Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]] spermatozoa.

* Aspirated [[Oogenesis - Anatomy & Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_- Anatomy & Physiology#Capacitation|capacitated]] spermatozoa.

** Could also be cultured in a 'nurse animal' in vivo.

** Could also be cultured in a 'nurse animal' in vivo.

* Embryos are incubated for a further period to the appropriate stage for transfer.

* Embryos are incubated for a further period to the appropriate stage for transfer.