Aspartate aminotransferase

Introduction

AST is not liver specific and is found in most tissues. Activity is relatively high in liver, skeletal and cardiac muscle. Significant increases may occur with liver or muscle damage or with haemolysis (in vitro or delayed removal of serum from a clot may cause mild to moderate increases). It is found in the cytosol and mitochondria of hepatocytes and is released into the blood due to hepatocellular damage and necrosis. In liver disease increases in AST usually parallel ALT. Therefore elevated AST and CK with normal ALT are more suggestive of muscle damage. There is no real advantage in measuring AST rather than ALT. The half life is about 12 hours in the dog, less in cats and 7 to 8 days in horses.

Aspartate aminotranferase (AST=GOT) is present in all body tissues but higher concentrations are found in the muscle and liver. Elevated values are suggestive of tissue damage especially involving liver or muscle. In association with CPK to rule out muscle damage it is a useful test in lizards for hepatocellular damage. Generalised diseases such as septicaemias may also elevate AST.

Small Animals

Causes of increased AST activity

• Hepatopathies (see ALT)
• Skeletal muscle damage, including overexertion/overactivity
• Cardiac muscle disorders including congestive heart failure

Complementary tests

Plasma AST activity is usually determined in conjunction with other tests of hepatocellular damage and skeletal muscle damage specifically ALP, ALT and GGT for hepatocellular damage and CK for skeletal muscle damage.

Equine

Common causes of increased AST activity

• Acute myopathy (exertional rhabdomyolysis, post exercise)
• Hepatopathies
• Other muscle disease for example, nutritional myodegeneration
• Haemolysis

Following an episode of azoturia, AST levels peak at 24-48 hours and decline slowly over 7-10 days returning to baseline levels by 10-21 days. This contrasts with CK which peaks after 6-12 hours and declines rapidly over 2 days returning to baseline levels by 3-4 days.

Complementary tests

Plasma AST activity should be assessed along with GLDH and GGT (hepatic disease) and CK (muscle damage including exertional rhabdomyolysis). Due to the different half-lives CK with AST is useful to assess recovery from acute myopathy.

Authors and References

NationWide Laboratories