Overview
- Organisms present in the soil, alimentary tract and faeces
- Endospores may be present in liver and may be reactivated to cause disease
- Neurotoxic clostridia, Clostridium tetani and Clostridium botulinum affect neuromuscular function but cause no tissue damage
- Histotoxic clostridia cause localised lesions in tissues and may cause toxaemia
- C. perfringens cause inflammatory lesions in the gastrointestinal tract and enterotoxaemias in sheep
Characteristics
- Large Gram-positive rods
- Obligate anaerobes
- Fermentative, catalase negative, oxidase negative
- Straight or slightly curved
- Motile by flagellae
- Require enriched media for growth
- Produce endospores which vary in shape and location and cause bulging of mother cell
Pathogenesis and pathogenicity
- Produce extracellular digestive enzymes and toxic substance known as exotoxins
- Exotoxins cause necrosis, haemolysis and death
- Collagenase, hyaluronidase and DNase enymes facilitate spread through tissues
Clostridium tetani
- Causes tetanus
- Acute, potentially fatal intoxication affecting many species
- Horses and man particularly susceptible; carnivores fairly resistant
- Found in horse faeces
- Characteristics:
- Terminal, spherical endospores give mother cells a drumstick appearance
- Enodospores resistant to boiling and chemicals but susceptible to autoclaving
- Swarming growth and haemolytic on blood agar
- Many serotypes but all produce same neurotoxin, tetanospasmin, therefore antibodies neutralise all
- Pathogenesis:
- Endospores introduced via damaaged tissues e.g. penetrating wounds
- Anaerobic conditions in the damaged tissue creates an anaerobic environment, allowing germination of spores
- Tetanospasmin made by bacteria replicating in damaged tissue
- Absorbed toxin affects neuromuscular junction distant from site of toxin production
- Neurotoxin binds irreversibly to ganglioside receptors on motor neurons and is transported to nerve cell body
- Toxins transported to terminals of inhibitory neurons where they block transmission of signals
- Spastic paralysis results
- Toxin can be blood-borne and bind to motor terminals throughout the body as well as in the CNS
- Clinical signs:
Clostridium botulinum
- The toxin also causes botulism
- Toxin produced in decaying organic matter absorbed from GIT into the blood and affects neuromuscular junction
- Implicated in equine grass sickness
Clostridium chauvei
- Causes gas gangrene, along with Clostridium septicum.
- Infects muscles giving black leg myositis
Clostridium novyi
- Causes gas gangrene and myositis.
- May be involved in cutaneous lesions
Clostridium perfringens
- Causes:
- Lamb dysentery
- Colitis X.
- Pulpy kidney disease
- C. perfringens type D only.
- Peritonitis in cattle
- Dysphagia in horses
- Gas gangrene
- Myositis
Clostridium septicum
- Causes gas gangrene and myositis
Clostridium sordelli
- Causes gas gangrene and myositis
Diagnosis
- Anaerobic transport medium
- Culture on blood agar enriched with yeast extract, vitamin K and haemin
- Anaerobic culture with hydrogen supplement and 5-10% carbon dioxide
- C. perfringens colonies are surrounded by a zone of double haemolysis
- Biochemical tests
- Toxins identified in body fluids by toxin neutralisation or protection tests in lab animals
- Fluorescent antibody tests for histotoxic clostridia
- ELISA, PCR for toxin detection