Semen Sexing - Anatomy & Physiology
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BACK TO REPRODUCTIVE SYSTEM
BACK TO REPRODUCTIVE TECHNOLOGIES
Principle
- X and Y chromosomes contain different quantities of DNA.
- X-bearing sperm contain 2.8-4.2% more DNA (species dependent) than Y-bearing sperm.
- This allows them to be sorted into two subpopulations.
- Separation requires the uptake of DNA stain/dye called Fluorochrome into living and dead sperm.
- X-bearing sperm take up more dye than Y-bearing sperm.
- Vital dyes emit light at a specific wavelength when excited or activated.
- X-bearing sperm will emit a larger signal than Y-bearing sperm.
- This allows the sperm to be sorted by flow cytometry.