Classical Swine Fever

Description

Classical swine fever is a highly contagious, haemorrhagic disease of swine which is caused by a Togavirus. Presentation may be actue, sub-acute, chronic or persistent, and the disease is indistinguishable in the field from African Swine Fever. Acutely, classical swine fever is characterised by severe depression, high fever and superficial and internal haemorrhages, with many cases resulting in death. Depression, anorexia and pyrexia are seen in chronic classical swine fever. Transplacental infection is also possible and results in persistently infected piglets.

Aetiology

The causative agent of classical swine fever is a small, enveloped virus of around 40nm diameter. The genome is comprised of single stranded RNA, which is positive sense and contains about 12,300 bases. The sequence of the genome is known, and codes four structural and seven non-structural proteins. The classical swine fever virus is relatively stable in excretions and in fresh meat products including ham, salami and other similar sausages. It is, however, easily inactivated by detergents, common disinfectants and heat.

Classical swine fever virus is a Togavirus within the Pestivirus genus of the Flaviviridae. As such, it is closely related to the bovine viral diarrhoea (BVD) virus of cattle, and the border disease virus of sheep.

Signalment

Domestic pigs and other swine of any age may become infected with classical swine fever.

Transmission and Pathogenesis

In field cases of CSFV, transmission is mainly oronasal by direct or indirect contact with infected pigs. Infected feed or pork products may also cause spread of disease, and transmission in semen can occur. Once the virus gains entry to the host an incubation period of around 7 days ensues, but this may vary from 4-10 days. Initially, virus infects the epithelial cells of the tonsillar crypts before spreading via the lymphatics to regional lymph nodes. From here, classical swine fever virus enters the blood stream and then replicates in the spleen, bone marrow and lymph nodes before spreading to further tissues. Replication in the endothelial cells of blood vessels leads to apoptosis, causing superficial and internal haemorrhages. CSFV also causes a thrombocytopenia which contributes to haemorrhage by impairing primary haemostasis. In acute CSF this angiopathy causes pig death in association with shock and the febrile response. Surviving swine go on to develop a chronic form of the disease where joint and enteric lesions are seen resulting from tissue infarction.

The outcome of transplacental infection of foetuses depends largely on the point of gestation and may result in abortions, stillbirths, mummifications, malformations or the birth of weak or persistently viraemic piglets. Although persistently infected piglets may be clinically normal at birth, they grow poorly, excrete virus over long periods and invariably die from CSF.

Diagnosis

Diagnosis is made on the basis of history, clinical signs and gross pathological lesions. In Britain, classical swine fever is notifiable to the local Animal Health Office. Following notification, the State Veterinary Service is responsible for visiting the suspect premises to confirm the diagnosis by laboratory testing.

Clinical Signs

Although the incubation period for classical swine fever is generally less than ten days, in the field it may take up to four weeks for clinical signs to become apparent in a population. Disease severity varies with virulence, immune status and the age of the animal: this means that although acute, chronic and congenital forms of the disease can be appreciated, there is no "classic" disease presentation.

In the acute form, animals are almost always pyrexic. In piglets the fever may exceed 40°, but in adults temperatures may be no higher than 39.5°. Lethargy, conjunctivitis, lymphomegaly may be seen, as well as respiratory signs and diarrhoea. Neurological signs such as gait abnormalities, incoordination and convulsions are also common. The most telling sign of classical swine fever is haemorrhage of the skin. These arise in the second or third week post-infection on the ear, tail, abdomen and medial aspect of the limbs and persist until death. CSF virus also causes severe leukopenia and immunosuppression, which can to secondary enteric or respiratory infections which may cause confusion by masking or overlapping the more typical signs of CSF. With increasing age of infected animals, the clinical signs of acute CSF become less specific and diagnosis more difficult. Alao, acute classical swine fever is indistinguishable from African swine fever and so care must be taken when formulating a diagnosis. Other differential diagnoses for acute CSF are erysipelas, PRRS, purpura haemorragica, PWMS, PDNS, Salmonellosis and Pasteurellosis. Classical swine fever should also be considered in any pyrexic enteric or respiratory disease case that is not responsive to antibiotics.

The chronic form of classical swine fever develops when pigs fail to mount an effective immune response to viral infection. Initially, the signs are similar to the acute form of the disease, but symptoms become less specific as the course progresses. For example, pigs may display chronic enteritis, loss of condition, lameness or intermittent pyrexia. In a herd, mortality may be increased or there may be large numbers of runty pigs. Although animals may survive some months after contracting chronic CSF, the disease is always eventually fatal and animals continue to shed virus until death.

The course of infection in older, breeding-age animals is often subclinical; however, CSFV is able to cross the placenta at any stage of pregnancy. The outcome of transplacental infection is highly dependent on the stage of gestation, and also virulence. During early pregnancy, transplacental CSFV infection may cause abortions, mummifications, congenital malformations or stillbirths. Infection occuring after 50-70 days gestation can lead to the birth of persistently viraemic piglets. These may appear clinically normal at birth, but grow poorly and occasionally show congenital tremor. Persistently infected piglets also shed virus until their inevitable death, acting as a reservoir for virus and making major contributions to the maintenance of infection in the population. It is therefore important to consider classical swine fever as a differential diagnosis of reduced fertility in addition to parvovirus, PRRS, leptospirosis and Aujeszky's disease.

Pathology

In acute classical swine fever, the major pathological change is multiple haemorrhages. This is seen as many purple blotches in the skin, and as sub-capsular bleeding in association with swelling and oedema in all lymph nodes. A "turkey egg" appearance to the kidneys is displayed, with haemorrhage varying from petechiae to ecchymoses. Haemorrhage may also be seen on any mucosal or serosal surface, including the urinary bladder and the larynx and epiglottis. The heart can be affected, and haemorrhage between other muscles is possible. The lungs are congested and haemorrhagic and often show bronchopneumonia, and straw-coloured fluid accumulates in the thoracic and abdominal cavities and the pericardial sac. A non-suppurative encephalitis can also feature.

The pathological changes of chronic classical swine fever are generally less typical, and organs and serosae usually lack haemorrhages. Necrotic, ulcerative lesions known as "button ulcers" are commonly seen in the ileum and rectum and at the ileocaecal valve in animals suffering chronic diarrhoea. Joint pathology is another frequent finding. However, the clinical signs of chronic CSF are non-specific and may vary according to secondary infections, and this is reflected in the pathological presentation of the disease.

The most common finding in cases of congenital classical swine fever is CNS pathology, particularly cerebellar hypoplasia.

Laboratory Tests

Laboratory testing is required to confirm a diagnosis of classical swine fever. As well as collection of tissues for histopathology, samples of tonsils, spleen, lymph nodes, kidney and distal ileum are taken for virus detection. Virus may be detected by fluorescent antibody detection, in situ hybridisation, immunoperoxidase staining or virus isolation. These methods are reviewed in Moennig¹ and briefly described below.

The gold standard laboratory test for CSFV is virus isolation in cell culture. In viraemic animals, virus may be isolated both from buffy coat cells and from supsensions of spleen, lymph node, tonsil, kidney or parotid salivary glands. Samples are incubated on cultures of porcine cells, and since classical swine fever virus is non-cytopathogenic, anti-CSFV antibodies are used to detect virus. Depsite good specificity and sensitivity, the virus isolation process takes around three days and is labour intesive and therefore costly.

Fluorescent antibody testing is less sensitive than virus isolation, abA rapid, though less sensitive test for CSFV is based on the demonstration of viral antigen in organ tissue sections using ¯uorescent antibodies. For the screening of large numbers of animals in herds suspect of being recently infected by CSFV, virus antigen capture enzyme-linked immunosorbent assays (AgC-ELISAs) may be used. This test is also less sensitive compared with virus isolation. Recently, detection of viral RNA has become an additional option for laboratory diagnosis (Paton et al., 2000b). In particular, the 5'nontranslated region of the genome has been used for ampli®cation by the reverse transcriptase polymerase chain reaction (RT-PCR). Subsequent nucleotide sequencing of the respective region allows discrimination between different CSFV isolates (Hofmann et al., 1994; Lowings et al., 1996; Greiser-Wilke et al., 1998). The EU/OIE Reference Laboratory for CSF in Hannover, Germany, keeps a large computer data base on CSF virus isolates including epidemiological and virus type information data (Greiser-Wilke et al., 2000). 5.2. Serology Considering the progress in antigen detection methods the importance of serology in the control of acute outbreaks has somewhat decreased. However, serological diagnosis of CSF is still important for surveys and the detection of hidden clusters of CSF, especially in wild boar. V. Moennig / Veterinary Microbiology 73 (2000) 93±102 97 The virus neutralisation test is the most sensitive and speci®c method for CSF antibody detection. Porcine serum samples are incubated with a CSF reference virus. In case the serum contains antibodies to CSFV the test virus will be neutralised. However, crossneutralising antibodies speci®c for ruminant pestivirus infections of pigs are often also registered by this test. Differential diagnosis for ruminant pestiviruses should therefore be carried out using a second neutralisation test using ruminant pestiviruses. The neutralisation test takes at least 2±3 days or longer if comparative testing is required and it is labour intensive. Large numbers of serum samples are, therefore, processed using ELISA tests. Positive or unclear results should be retested using the neutralisation test

Treatment

Control

• NOTIFIABLE disease
• Vaccination (live attenuated) in endemic countries:
  • Parts of EU are using vaccinated bait to control spread in wild boar population
  • Vaccination does not curtail spread: marker vaccine needed to distinguish virus exposure from vaccine-induced antibody

Links

• Defra - Classical Swine Fever

References

1. Moennig, V (2000) Introduction to classical swine fever: virus, disease and control policy, Veterinary Microbiology, 73, 93-102.
2. Moennig, V et al (2003) Clinical Signs and Epidemiology of Classical Swine Fever: A Review of New Knowledge, The Veterinary Journal, 165, 11-20.
3. Paton, DJ and Greiser-Wilke, I (2003) Classical swine fever – an update, Research in Veterinary Science, 75, 169-178.