Line 23: |
Line 23: |
| *Non-motile | | *Non-motile |
| *Catalase negative, oxidase negative, coagulase positive | | *Catalase negative, oxidase negative, coagulase positive |
− | *Isolates from acute infections produce smooth colonies
| |
− | *Isolates from chronic infections produce rough colonies
| |
| *Grow on nutrient agar; growth enhanced by addition of blood or serum | | *Grow on nutrient agar; growth enhanced by addition of blood or serum |
| *Small colonies with incomplete haemolysis in 48 hours | | *Small colonies with incomplete haemolysis in 48 hours |
− | *Hydrogen sulphide sormed along stab line in TSI agar | + | *Hydrogen sulphide formed as black central line in TSI agar |
| *Growth over wide rangs of temperatures and pH | | *Growth over wide rangs of temperatures and pH |
| + | |
| + | |
| + | ===Identification and diagnosis=== |
| + | |
| + | *Isolates from acute infections produce smooth colonies; 1.5mm diamteter, convex and circular with even edges |
| + | *Isolates from chronic infections produce rough colonies; larger, flat and opaque colonieswith irregular edges |
| + | *Biochemical tests |
| + | *Serotypying according to a heat-stable peptidoglycan extracted from the cell wall |
| + | *Virulence testing in lab animals |
| + | *PCR for detection of virulent isolates |
| + | |
| + | |
| + | ===Pathogenesis and pathogenicity=== |
| + | |
| + | *Route of infection: ingestion from pig faeces |
| + | *Entry via tonsils, skin or mucous membranes |
| + | *Capsule prevents phagocytosis |
| + | *Adhere to endothelial cells |
| + | *Produce neurominidase which enhances cell penetration |
| + | *Septicaemia with vascular damage |
| + | *Swelling of endothelial cells, monocyte adherence to vascular walls and hyaline microthrombus formation |
| + | *Bacteria localise in synovia of joints and on heart valves during bacteraemia and cause chronic lesions |
| + | *Host immune response to persistent bacterial antigens causes long-term damage to the joints |
| + | |
| + | |
| + | ===Clinical infections=== |