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Dysfunction of serotonergic neurotransmitter system in dogs has been linked to a number of problems, including aggression<ref>Rosado, B., Garcia-Belenguer, S., Leon, M., et al. Blood concentrations of serotonin, cortisol, and dehydroepiandrosterone in aggressive dogs. Appl Anim Behav Sci 2010; 123:124-30</ref>. In one study feeding dogs a diet with a lower protein content decreased territorial aggression<ref>Dodman, N.H., Reisner, I., Shuster, L., et al. Effect of dietary protein content on behaviour of dogs. J Am Vet Med Assoc 1996; 208:376-9</ref>, although other types of aggression seemed to be uninfluenced.  
 
Dysfunction of serotonergic neurotransmitter system in dogs has been linked to a number of problems, including aggression<ref>Rosado, B., Garcia-Belenguer, S., Leon, M., et al. Blood concentrations of serotonin, cortisol, and dehydroepiandrosterone in aggressive dogs. Appl Anim Behav Sci 2010; 123:124-30</ref>. In one study feeding dogs a diet with a lower protein content decreased territorial aggression<ref>Dodman, N.H., Reisner, I., Shuster, L., et al. Effect of dietary protein content on behaviour of dogs. J Am Vet Med Assoc 1996; 208:376-9</ref>, although other types of aggression seemed to be uninfluenced.  
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In both dogs and cats fed a L-tryptophan supplement, lower levels of behaviours related to stress and fewer signs of anxiety were seen in one study<ref>Da Graca Pereira, G., Fragoso, S., L-tryptophan supplementation and its effect of multi-housed cats and working dogs. Proceedings of the 2010 European Veterinary Behaviour Meeting. Hamburg, 2010, 30-35</ref><ref name="Kato">Kato, M., Miyaji, K., Ohtani, N., et al. Effects of prescription diet on dealing with stressful situations and performance of anxiety-related behaviours in privately owned anxious dogs. 2012; 7:21-6</ref>.  
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In both dogs and cats fed a L-tryptophan supplement, lower levels of behaviours related to stress and fewer signs of anxiety were seen in one study, but this was not in a peer-reviewed journal<ref>Da Graca Pereira, G., Fragoso, S., L-tryptophan supplementation and its effect of multi-housed cats and working dogs. Proceedings of the 2010 European Veterinary Behaviour Meeting. Hamburg, 2010, 30-35</ref>. In another study, an axiolytic effect was fond, but the diet contained alpha-casozepine as well as l-tryptophan, so any effect cannot be ascribed to amino acid acid supplementation alone<ref name="Kato">Kato, M., Miyaji, K., Ohtani, N., et al. (2012) Effects of prescription diet on dealing with stressful situations and performance of anxiety-related behaviours in privately owned anxious dogs. Journal of Veterinary Behavior: Clinical Applications and Research. 7(1). 21–26.</ref>. A randomised double-blinded, placebo-controlled study showed no effect of an l-tryptophan enriched diet on behaviour or salivary cortisol in dogs, despite measurable increases in plasma levels of the amino acid<ref name="Kato">Kato, M., Miyaji, K., Ohtani, N., et al. (2012) Effects of prescription diet on dealing with stressful situations and performance of anxiety-related behaviours in privately owned anxious dogs. Journal of Veterinary Behavior: Clinical Applications and Research. 7(1). 21–26.</ref>Bosch, G., Beerda, B., Beynen, A.C., van der Borg, J.A.M.,  b, van der Poel, A.F.B., Hendriks, W.H.,  (2009) Dietary tryptophan supplementation in privately owned mildly anxious dogs. Applied Animal Behaviour Science. 121. 197–205</ref>.
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*A higher carbohydrate diet is thought to be linked to tryptophan levels in the brain increasing. This as previously mentioned can be calming, which may in turn decrease the potential for aggressive behaviour. It must be noted however, that if the levels of carbohydrates are increased by reducing protein levels, the reduced protein may be the factor which causes this effect.
 
*A higher carbohydrate diet is thought to be linked to tryptophan levels in the brain increasing. This as previously mentioned can be calming, which may in turn decrease the potential for aggressive behaviour. It must be noted however, that if the levels of carbohydrates are increased by reducing protein levels, the reduced protein may be the factor which causes this effect.
  
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