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Cellular Fatty Change (view source)
Revision as of 14:03, 24 December 2014
, 14:03, 24 December 2014→Histological Appearance of Fatty Change
===Histological Appearance of Fatty Change===
===Histological Appearance of Fatty Change===
* The fat either appears as globules or is contained in varying sizes of vacuoles in the cytoplasm.
* Fat appears as clear colourless vacuoles of varying sizes in the cell cytoplasm.
** In the heart, fat appears as groups of tiny vacuoles dispersed along the myofibrils.
** In the heart, fat appears as groups of tiny vacuoles dispersed along the myofibrils.
** In the [[Liver - Anatomy & Physiology|liver]] and kidney, vacuoles tend to coalesce to form larger ones.
** In the [[Liver - Anatomy & Physiology|liver]] and kidney, vacuoles tend to coalesce to form larger ones.
*** One or more large globules may fill the cytoplasm.
*** One or more large vacuoles may fill the cytoplasm.
**** The nucleus is displaced to the periphery of the cell.
**** The nucleus is displaced to the periphery of the cell.
* The nucleus remains normal.
* The nucleus remains normal.
** Stains commonly used include Sudan III, Sudan IV, and Oil Red O.
** Stains commonly used include Sudan III, Sudan IV, and Oil Red O.
*** Stain fat varying shades of orange to red.
*** Stain fat varying shades of orange to red.
** Sections must be prepared differently to the routine paraffin embedding (used e.g. in H&E staining).
** The strong solvents used in paraffin embedding dissolve the fat out of the cell.
*** Sections must therefore be prepared differently to the routine paraffin embedding (used e.g. in H&E staining).
*** When staining for fat, the tissue to be examined is frozen and sectioned in a cryostat before being stained.
*** When staining for fat, the tissue to be examined is frozen and sectioned in a cryostat before being stained.
**** These sections are more than twice as thick as those attained by sectioning paraffin blocks
**** These sections are more than twice as thick as those attained by sectioning paraffin blocks
***** There may be some overlap of cells on the section.
***** There may be some overlap of cells on the section.