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* Chimerism (XX/XY) of [[Haematopoiesis_-_Anatomy_%26_Physiology|haematopoeitic cells]].  Blood tests can be used to demonstrate different chromosome complement in nucleated leukocytes and presence of two blood groups in each twin.
 
* Chimerism (XX/XY) of [[Haematopoiesis_-_Anatomy_%26_Physiology|haematopoeitic cells]].  Blood tests can be used to demonstrate different chromosome complement in nucleated leukocytes and presence of two blood groups in each twin.
 
* Rectal palpation and vaginoscopy reveal a shortened vagina  
 
* Rectal palpation and vaginoscopy reveal a shortened vagina  
** In calves 1-4 weeks old, the vagina is normally 13-15cm in length compared to 5-6cm in a freemartin.
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** In calves 1-4 weeks old, the [[/Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vagina]] is normally 13-15cm in length compared to 5-6cm in a freemartin.
* Vulva and vestibule patent but vestibule or vagina is blind-ended - can be checked using speculum or probe.
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* Vulva and vestibule patent but [[/Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vestibule or vagina]] is blind-ended - can be checked using speculum or probe.
    
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