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'''Solid VS Fluid-phase'''
'''Solid VS Fluid-phase'''
[[Image:800px-Microtiter plate.jpg|thumb|right|150px|96-well microtiter plate, Copyright Jeffrey M. Vinocur 2006]]
An important consideration is the medium in which the assay is carried out, largely dependent on what is being tested:
An important consideration is the medium in which the assay is carried out, largely dependent on what is being tested:
*Solid-phase (''surface of protein-binding material, e.g. plastic'')
*Solid-phase (''surface of protein-binding material, e.g. plastic'')
**Easier to perform and more sensitive
**Easier to perform and more sensitive
**The most widely used solid-phase is the 96-well microtiter plater, manufactured as PVC flexible plates or polystyrene rigid plates
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===Non-competitive ELISA===
===Non-competitive ELISA===