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***One preparation of secondary antibody can be used to test many sera
 
***One preparation of secondary antibody can be used to test many sera
 
***By using a mixture of primary antibodies, it is possible to detect the relative expressions of different antigens in the same cell
 
***By using a mixture of primary antibodies, it is possible to detect the relative expressions of different antigens in the same cell
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***Quite often loss of antibody is sustained during the conjugation- in the indirect method the primary antibodies do not need to be conjugated, so this limiting factor is reduced.
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==Applications==
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*Immunofluorescence is often used to identify populations of cells, and was successfully employed in the identification of the CD4+ and CD8+ subpopulations of T cells
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*Identifying bacterial species
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*Detecting antigen-antibody complexes in autoimmune diseases
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*Detecting complement components in tissues
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*Localising hormones, antigens and other cellular products in tissue sampler and subcellular compartments
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*Mapping the molecular architecture of tissues in relation to gross anatomy
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==Developments==
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===Confocal microscopy===
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