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* The age at which [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] first occurs.
 
* The age at which [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] first occurs.
* Manual or visual validation is required. This can be accomplished by palpation of the ovary per rectum in animals that are large enough to permit insertion of a hand/arm into the rectum (cow and camelids).
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* Manual or visual validation is required. This can be accomplished by palpation of the [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|ovary]] per rectum in animals that are large enough to permit insertion of a hand/arm into the rectum (cow and camelids).
 
* Ultrasound can be used most successfully in the mare to determine ovarian status.
 
* Ultrasound can be used most successfully in the mare to determine ovarian status.
* When [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] has occured, a soft depression on the surface of the ovary can be palpated.
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* When [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] has occured, a soft depression on the surface of the [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|ovary]] can be palpated.
* In smaller animals (sow, ewe, bitch, queen) surgical procedures are necessary to allow visualisation of the ovary in order to determine [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]].  In addition laparoscopic observation can be used to determine when [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] occurs.
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* In smaller animals (sow, ewe, bitch, queen) surgical procedures are necessary to allow visualisation of the [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|ovary]] in order to determine [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]].  In addition laparoscopic observation can be used to determine when [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] occurs.
* All techniques require frequent observation of the ovary, so although age at [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] is a good criterion for puberty, it is difficult to determine.
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* All techniques require frequent observation of the [[Female_Reproductive_Tract_-The_Ovary_-_Anatomy_%26_Physiology|ovary]], so although age at [[The_Ovary_-_Ovulation_-_Anatomy_%26_Physiology|ovulation]] is a good criterion for puberty, it is difficult to determine.
    
== Age at which the Female can Support Pregnancy without Deleterious Effects ==
 
== Age at which the Female can Support Pregnancy without Deleterious Effects ==
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