Changes

===Wet mount===

===Wet mount===

Make a direct wet mount and examine under low power for nematode larvae and high power for protozoa.

Make a direct wet mount and examine under low power for nematode larvae and high power for protozoa.

*Water preparation - nematode ova and moving flagellates (concentrated saline may destroy flagellates immediately) iodine stain (e.g. Lugol's) or eosin - identification of encysted entamoeba.  

*Water preparation - nematode ova and moving flagellates (concentrated saline may destroy flagellates immediately).

*Gram stain - Though they are frequently recovered from clinically healthy captive reptiles, most pathogenic infections are caused by Gram negative pathogens e.g. Pseudomonas, Aeromonas, Proteus, Salmonella.

*Iodine stain (e.g. Lugol's) or eosin - identification of encysted entamoeba.  

*Gram stain - Though they are frequently recovered from clinically healthy captive reptiles, most pathogenic infections are caused by Gram negative pathogens e.g. ''Pseudomonas'', ''Aeromonas'', ''Proteus'', ''Salmonella''.

 

===Flotation===

===Flotation===

The faecal material is mixed with the high specific gravity flotation fluid. The debris is removed with a filter and material that is less dense than the flotation fluid, such as nematode ova, will rise to the top. This can then be examined on a microscope slide.

The faecal material is mixed with the high specific gravity flotation fluid. The debris is removed with a filter and material that is less dense than the flotation fluid, such as nematode ova, will rise to the top. This can then be examined on a microscope slide.