Changes

===Laboratory Tests===

===Laboratory Tests===

Laboratory testing is required to confirm a diagnosis of classical swine fever. As well as collection of tissues for histopathology, samples of tonsils, spleen, lymph nodes, kidney and distal ileum are taken for virus detection. Virus may be detected by fluorescent antibody detection, ''in situ'' hybridisation, immunoperoxidase staining or virus isolation.

Laboratory testing is required to confirm a diagnosis of classical swine fever. As well as collection of tissues for histopathology, samples of tonsils, spleen, lymph nodes, kidney and distal ileum are taken for virus detection. Virus may be detected by fluorescent antibody detection, ''in situ'' hybridisation, immunoperoxidase staining or virus isolation. These methods are reviewed in Moennig¹ and briefly described below.

Although much progress had been made in the development of new methods for the

The gold standard laboratory test for CSFV is virus isolation in cell culture. In viraemic animals, virus may be isolated both from buffy coat cells and from supsensions of spleen, lymph node, tonsil, kidney or parotid salivary glands. Samples are incubated on cultures of porcine cells, and since classical swine fever virus is non-cytopathogenic, anti-CSFV antibodies are used to detect virus. Depsite good specificity and sensitivity, the virus isolation process takes around three days and is labour intesive and therefore costly.

direct detection of CSFV, the `gold standard' is still the isolation of the virus in cell culture.

 

CSFV can be isolated from buffy coat cells or organ suspensions of viraemic animals.

Fluorescent antibody testing is less sensitive than  virus isolation, abA rapid, though less sensitive test for CSFV

Suitable organs are spleen, tonsils, lymph nodes, parotid glands and kidneys (Anonymous,

1980, 1996). The samples are incubated on susceptible cell cultures of porcine origin. Since

CSFV is noncytopathogenic, CSFV speci®c antibodies are used for detecting the virus in

cell culture. Differentiation of the CSFV from ruminant pestiviruses is usually done using

monoclonal antibodies (mAbs) (Anonymous, 1980, 1996; Cay et al., 1989). Virus isolation

takes about 3 days and is labour intensive. A rapid, though less sensitive test for CSFV

is based on the demonstration of viral antigen in organ tissue sections using ¯uorescent

is based on the demonstration of viral antigen in organ tissue sections using ¯uorescent

antibodies. For the screening of large numbers of animals in herds suspect of being

antibodies. For the screening of large numbers of animals in herds suspect of being