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Toxoplasmosis - Cat and Dog (view source)
Revision as of 09:56, 13 August 2010
, 09:56, 13 August 2010→Laboratory Tests
===Laboratory Tests===
===Laboratory Tests===
Demonstration of ''Toxoplasma gondii'' in the tissues with associated inflammation is required for the definitive diagnosis of clinical toxoplasmosis. This is usually achieved post-mortem, but ante-mortem testing of tissues and effusions for bradyzoites or tachyzoites is possible. Many normal animals possess ''Toxoplasma gondii''-specifice antibodies in the serum , aqueous humour and CSF, and so serology alone is not appropriate for diagnosis of toxoplasmosis. However, a combination of various diagnostic procedures can be used to build a presumptive diagnosis. Cytology is of some use in the diagnosis of toxoplasmosis. Tachyzoites may be seen in the blood, cerebrospinal fluid, peritoneal and pleural effusions or transtracheal washes from clinically ill animals<sup>1</sup>. CSF analysis may show elevated protein levels. In addition to using cytology to demonstrate T gondii
Demonstration of ''Toxoplasma gondii'' in the tissues with associated inflammation is required for the definitive diagnosis of clinical toxoplasmosis. This is achieved most easily post-mortem, but ante-mortem testing of tissues and effusions for bradyzoites or tachyzoites is possible. For example, tachyzoites may be seen in the blood, cerebrospinal fluid, peritoneal and pleural effusions or transtracheal washes from clinically ill animals. PCR, tissue culture and animal inoculation techniques can also be used to detect ''Toxoplasma gondii'' in blood, aqueous humour or CSF<sup>1</sup>.
Many normal animals possess ''Toxoplasma gondii''-specifice antibodies in the serum , aqueous humour and CSF, and so serology alone is not appropriate for diagnosis of toxoplasmosis. However, a combination of various diagnostic procedures can be used to build a presumptive diagnosis.
CSF analysis may show elevated protein levels.
Tissue biopsy sections
can be assessed for the presence of T gondii by haematoxylin
can be assessed for the presence of T gondii by haematoxylin
and eosin (H&E) staining, immunohistochemical
and eosin (H&E) staining, immunohistochemical