Changes

===Laboratory Tests===

===Laboratory Tests===

Demonstration of ''Toxoplasma gondii'' in the tissues with associated inflammation is required for the definitive diagnosis of clinical toxoplasmosis. This is achieved most easily post-mortem, but ante-mortem testing of tissues and effusions for bradyzoites or tachyzoites is possible. For example, tachyzoites may be seen in the blood, cerebrospinal fluid, peritoneal and pleural effusions or transtracheal washes from clinically ill animals. PCR, tissue culture and animal inoculation techniques can also be used to detect ''Toxoplasma gondii'' in blood, aqueous humour or CSF¹.

Demonstration of ''Toxoplasma gondii'' in the tissues with associated inflammation is required for the definitive diagnosis of clinical toxoplasmosis. For example, tachyzoites may be seen in blood, cerebrospinal fluid, peritoneal and pleural effusions, aqueous humour or transtracheal washes from clinically ill animals. ''Toxoplasma gondii'' may also be detected in these samples using PCR, tissue culture or animal inoculation techniques¹. These methods may be employed on tissue biopsies too, as well as examination under haematoxylin and eosin or immunohistochemical staining. Immunohistochemistry is preferred to H&E because it is specific for ''T. gondii''. Demonstration of the organism is often most easily achieved post-mortem, as the size of the sample is not restrictive to the likelyhood of seeing ''T.gondii''. Even then, the pathogenesis of disease may be primarily immune-mediated in some cases and so there may be relatively few organisms to find. This is particularly true of opthalmic disease.

* Clinical signs of disease referable to toxoplasmosis;

* Clinical signs of disease referable to toxoplasmosis;