Brucella species

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• B. abortus occasionally in arthritis of cattle

• Isolated from closed cases of Poll Evil and Fistulous Withers

Overview

• Important zoonoses worldwide
• Cause chronic granulomatous diseases

Characteristics

• Small, non-moltile, Gram negative coccobacilli
• Facultatice intracellular pathogens

• 6 species

Pathogenesis and pathogenicity

• Penetrate nasal, oral or pharyngeal mucosa
• Phagocytosed and carried to regional lymph nodes
• Survive and multiply in cells of the reticulo-endothelial system
• Inhibit lysosome-phagosome fusion
• Lymph nodes enlarge (lymphatic and lymphoreticular hyperplasia) and inflammation is induced
• Surviving organisms spread to other organs (liver, spleen, placenta) and cause granulomatous reactions
• Eythritol is a growth stimulant and attracts the bacteria to the bovine placenta
• Infection of foetus and abortion

Control

• Serological testing of milk (Milk Ring Test) and beef cattle (Rose Bengal Plate Test)
• Serological tests detect anti-lipopolysaccharide antibodies
• LPS antigen present in virulent as well as some vaccine strains therefore vaccination may confuse serological testing
• False positives due to cross-reaction with LPS in other bacteria
• Vaccination:
  • Strain S19, a live vaccine, stimulating a cell-mediated immune response; vaccination of young animals; interferes with serological testing
  • Newer RB51 vaccine has no LPS O-antigen therefore not detcted by serological tests
  • Live attenuated Brucella melitensis vaccine to protect sheep and goats against B. melitensis
• Test and slaughter program has eradicated Brucellosis in the UK