Changes

Diagnosis can be made on the above clinical signs and '''decreases in haematocrit''' from normal ranges (32-37.5%) to '''below 27%''' and increases in the amount of [[Haematopoiesis - Overview|immature blood cells]].  

Diagnosis can be made on the above clinical signs and '''decreases in haematocrit''' from normal ranges (32-37.5%) to '''below 27%''' and increases in the amount of [[Haematopoiesis - Overview|immature blood cells]].  

''' Virus isolation''' can confirm diagnosis of disease but growth of CAV in cell cultures can be difficult. Levels of infection can be estimated by the detection of raising antibody titres and many diagnostic tests have been developed that include  immunoperoxidase staining, [[ELISA testing|ELISA]]<ref name=" Todd et al., 1999">Todd, D., Mawhinney, K.A., Graham, D.A., Scott, A.N.J.,(1999) '''Development of a blocking enzyme-linked immunosorbent assay for the serological diagnosis of chicken anaemia virus'''. ''Journal of Virological Methods'', 82(2):177-184; 17 ref. </ref>, PCR, dot blot hybridisation and indirect [[immunofluorescence]]<ref name=" Sun et al., 1999">Sun, W., Wu, Z.Q., Hu, Q.H., Li,  S.X., Li, G., (1999) ''' Preliminary research on the diagnosis of chicken infectious anaemia by PCR, dot-blot-hybridization assay and indirect immunofluorescence assay'''. '''Journal of Nanjing Agricultural University'', 22(3):69-72. </ref>.

''' Virus isolation''' can confirm diagnosis of disease but growth of CAV in cell cultures can be difficult. Levels of infection can be estimated by the detection of raising antibody titres and many diagnostic tests have been developed that include  immunoperoxidase staining, [[ELISA testing|ELISA]]<ref name=" Todd et al., 1999">Todd, D., Mawhinney, K.A., Graham, D.A., Scott, A.N.J.,(1999) '''Development of a blocking enzyme-linked immunosorbent assay for the serological diagnosis of chicken anaemia virus'''. ''Journal of Virological Methods'', 82(2):177-184; 17 ref. </ref>, PCR and indirect [[immunofluorescence]]<ref name=" Sun et al., 1999">Sun, W., Wu, Z.Q., Hu, Q.H., Li,  S.X., Li, G., (1999) ''' Preliminary research on the diagnosis of chicken infectious anaemia by PCR, dot-blot-hybridization assay and indirect immunofluorescence assay'''. '''Journal of Nanjing Agricultural University'', 22(3):69-72. </ref>.

Post mortem finding include severe atrophy of the lymphoid organs. The thymus, bursa of Fabricius, and to a lesser extent the spleen are all affected by a depletion of lymphocytes and sequential hyperplasia of reticular cells. Common finding include haemorrhages throughout the skeletal muscle and subcutaneous tissue and pale watery bone marrow. Severe aplasia of the bone marrow occurs and haematopoietic cells are replaced with adipose tissue, giving the bone marrow its watery texture and characteristic change in colour from red to yellow.

Post mortem finding include severe atrophy of the lymphoid organs. The thymus, bursa of Fabricius, and to a lesser extent the spleen are all affected by a depletion of lymphocytes and sequential hyperplasia of reticular cells. Common finding include haemorrhages throughout the skeletal muscle and subcutaneous tissue and pale watery bone marrow. Severe aplasia of the bone marrow occurs and haematopoietic cells are replaced with adipose tissue, giving the bone marrow its watery texture and characteristic change in colour from red to yellow.