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====Description====
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==Description==
 
'''NOTIFIABLE''' and '''ZOONOTIC''' infectious mosquito-borne diseases of equidae affecting the central nervous system (CNS).  They include:
 
'''NOTIFIABLE''' and '''ZOONOTIC''' infectious mosquito-borne diseases of equidae affecting the central nervous system (CNS).  They include:
 
*Eastern Equine Encephalitis (EEE)
 
*Eastern Equine Encephalitis (EEE)
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*Venezuelan Equine Encephalitis (VEE)
 
*Venezuelan Equine Encephalitis (VEE)
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====Aetiology====
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==Aetiology==
 
[[Equine Encephalitis Virus|See this page for details of the causal pathogens]].  Some of the virus strains can infect swine, poultry and other farmed birds including quail and ratites.  Isolated cases have also been noted in cattle, sheep and non-domestic ungulates.  Some strains are potential agents of biowarfare or bioterrorism (Steele and Twenhafel, 2010).
 
[[Equine Encephalitis Virus|See this page for details of the causal pathogens]].  Some of the virus strains can infect swine, poultry and other farmed birds including quail and ratites.  Isolated cases have also been noted in cattle, sheep and non-domestic ungulates.  Some strains are potential agents of biowarfare or bioterrorism (Steele and Twenhafel, 2010).
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====Epidemiology====
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==Epidemiology==
=====(1) Distribution=====
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===Distribution===
 
Togaviral encephalitis in equids is largely confined to the Western Hemisphere.  Venezuelan EEV can cause large outbreaks of disease over extensive geographical areas in both humans and horses.  Spread of this virus into Central America has had disasterous consequences with epidemics as far north as Texas. Climatic conditions and interventions that support vector populations, such as irrigation, greatly influence the geographical spread of the disease.  EEE has been recorded across the United States, but mostly in the Southeastern States. As its names suggests, WEE has a predilection for the Western states which have been subject to significant outbreaks in the past.  A regional alteration in virulence has been proposed for the steep decline in clinical case numbers observed in this area.  A lag phase of 2-5weeks is commonly observed between horse and human cases of WEE in a given locus.  Both are dead-end hosts for the virus.    A subtype of Western EEV, Highlands J virus, was isolated from the brain of a horse with encephalitis in Florida (Karabatsos ''et al''., 1988).
 
Togaviral encephalitis in equids is largely confined to the Western Hemisphere.  Venezuelan EEV can cause large outbreaks of disease over extensive geographical areas in both humans and horses.  Spread of this virus into Central America has had disasterous consequences with epidemics as far north as Texas. Climatic conditions and interventions that support vector populations, such as irrigation, greatly influence the geographical spread of the disease.  EEE has been recorded across the United States, but mostly in the Southeastern States. As its names suggests, WEE has a predilection for the Western states which have been subject to significant outbreaks in the past.  A regional alteration in virulence has been proposed for the steep decline in clinical case numbers observed in this area.  A lag phase of 2-5weeks is commonly observed between horse and human cases of WEE in a given locus.  Both are dead-end hosts for the virus.    A subtype of Western EEV, Highlands J virus, was isolated from the brain of a horse with encephalitis in Florida (Karabatsos ''et al''., 1988).
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=====(2) Transmission=====
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===Transmission===
 
Transfer is '''vector'''-mediated, primarily via '''[[Culicidae|mosquito]] salivary transfer'''.  WEE and VEE may also be transmitted horse to horse through nasal secretions.  This mode of transmission is less likely, despite the fact that high concentrations of VEE virus are found in ocular and nasal discharges from infected horses.  The viraemic phase ends when nervous signs develop and is important for disease amplification.  Amplification from horses is likely only with VEE virus, in association with a relatively high and potentially persistent viraemia.  Similarly, zoonotic spread is unlikely for Eastern and Western equine encephalitis, but has been noted with VEE.
 
Transfer is '''vector'''-mediated, primarily via '''[[Culicidae|mosquito]] salivary transfer'''.  WEE and VEE may also be transmitted horse to horse through nasal secretions.  This mode of transmission is less likely, despite the fact that high concentrations of VEE virus are found in ocular and nasal discharges from infected horses.  The viraemic phase ends when nervous signs develop and is important for disease amplification.  Amplification from horses is likely only with VEE virus, in association with a relatively high and potentially persistent viraemia.  Similarly, zoonotic spread is unlikely for Eastern and Western equine encephalitis, but has been noted with VEE.
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=====(3) Seasonal Incidence=====
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===Seasonal Incidence===
 
The disease is not directly contagious between horses and humans but occurs sporadically in both species from mid-summer to late autumn - during the height of the vector season.  Case numbers peak in June to November in temperate climates.  The vector season is longer in warmer climates, where the disease period is prolonged.  Global warming may promote more outbreaks in historically colder climates.
 
The disease is not directly contagious between horses and humans but occurs sporadically in both species from mid-summer to late autumn - during the height of the vector season.  Case numbers peak in June to November in temperate climates.  The vector season is longer in warmer climates, where the disease period is prolonged.  Global warming may promote more outbreaks in historically colder climates.
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=====(4) Epidemics=====
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===Epidemics===
 
Outbeak prediction to date has been inaccurate, implying that other, unidentified factors may be in operation.  However, some epidemic requirements are beyond question.  Adequate amounts of infective virus, sufficent vectors, infected sylvatic hosts and susceptible terminal hosts, and finally, appropriate reservoirs, are all crucial.
 
Outbeak prediction to date has been inaccurate, implying that other, unidentified factors may be in operation.  However, some epidemic requirements are beyond question.  Adequate amounts of infective virus, sufficent vectors, infected sylvatic hosts and susceptible terminal hosts, and finally, appropriate reservoirs, are all crucial.
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====Pathogenesis====
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==Pathogenesis==
 
Upon entry to the host, viruses multiply in the muscle, enter the lymphatic circulation and localize in lymph nodes.  In macrophages and neutrophils viral replication leads to shedding and significant clearance of viral particles.  No further clinical signs develop if this clearance is successful.  Erythrocyte and leukocyte absorption are used to circumvent the immune defences of the host.  After incomplete elimination, residual virus infects endothelial cells and accumulates in highly vascular organs such as the liver and spleen.  In these organs, viral replication produces circulating virus and a second viraemic period, typically associated with early clinical signs.  Neuroinvasion and replication occurs within a week.  An incubation period of 7-21days has been demonstrated after experimental infection with Eastern or Western EEV, but the incubtion is often shorter for EEE compared with that of WEE.
 
Upon entry to the host, viruses multiply in the muscle, enter the lymphatic circulation and localize in lymph nodes.  In macrophages and neutrophils viral replication leads to shedding and significant clearance of viral particles.  No further clinical signs develop if this clearance is successful.  Erythrocyte and leukocyte absorption are used to circumvent the immune defences of the host.  After incomplete elimination, residual virus infects endothelial cells and accumulates in highly vascular organs such as the liver and spleen.  In these organs, viral replication produces circulating virus and a second viraemic period, typically associated with early clinical signs.  Neuroinvasion and replication occurs within a week.  An incubation period of 7-21days has been demonstrated after experimental infection with Eastern or Western EEV, but the incubtion is often shorter for EEE compared with that of WEE.
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====Signalment====
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==Signalment==
 
Unvaccinated adult horses and '''other equids''' (for VEE in donkeys [[Venezuelan Equine Encephalomyelitis (VEE) - Donkey|see here]]) are at risk in areas with suitable vectors.  Vaccinated horses can still develop the disease, particularly if they are young or old.
 
Unvaccinated adult horses and '''other equids''' (for VEE in donkeys [[Venezuelan Equine Encephalomyelitis (VEE) - Donkey|see here]]) are at risk in areas with suitable vectors.  Vaccinated horses can still develop the disease, particularly if they are young or old.
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====Clinical Signs====
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==Clinical Signs==
    
Worse in unvaccinated horses.  Neurological signs may be assymmetrical.   
 
Worse in unvaccinated horses.  Neurological signs may be assymmetrical.   
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=====(1) EEE and WEE=====
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===EEE and WEE===
 
Following an incubation period of 7-21days, an initial pyrexia and mild depression are short-lived and often missed.  The acute phase of the disease presents with mild to severe pyrexia, anorexia and stiffness, lasting up to 5 days.  During this time, the horse is viraemic and capable of amplifying the disease.  The fever may then fluctuate with neurological derangements appearing a few days post-infection.  These changes indicate disease progression, which occurs more frequently with EEE (the most virulent of the three serotypes).  Any of the following may be observed:
 
Following an incubation period of 7-21days, an initial pyrexia and mild depression are short-lived and often missed.  The acute phase of the disease presents with mild to severe pyrexia, anorexia and stiffness, lasting up to 5 days.  During this time, the horse is viraemic and capable of amplifying the disease.  The fever may then fluctuate with neurological derangements appearing a few days post-infection.  These changes indicate disease progression, which occurs more frequently with EEE (the most virulent of the three serotypes).  Any of the following may be observed:
 
*conscious proprioceptive deficits
 
*conscious proprioceptive deficits
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*recumbency for 1-7 days followed by death
 
*recumbency for 1-7 days followed by death
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=====(2) VEE=====
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===VEE===
 
Clinical signs may resemble those of WEE and EEE or may vary:
 
Clinical signs may resemble those of WEE and EEE or may vary:
 
*pyrexia peaks early and persists throughout the disease course
 
*pyrexia peaks early and persists throughout the disease course
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*other signs: abortion, oral ulceration, pulmonary haemorrhage, epistaxis
 
*other signs: abortion, oral ulceration, pulmonary haemorrhage, epistaxis
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====Diagnosis====
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==Diagnosis==
 
Presumptive based on epidemiology and clinical signs.  Definitive diagnosis requires virus identification, serological tests and/or post-mortem examination.   
 
Presumptive based on epidemiology and clinical signs.  Definitive diagnosis requires virus identification, serological tests and/or post-mortem examination.   
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=====(1) Laboratory Tests=====
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===Laboratory Tests===
======Virus Identification======
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====Virus Identification====
 
Viruses can be identified by complement fixation, immunofluorescence, PCR, ELISA, or virus isolation.
 
Viruses can be identified by complement fixation, immunofluorescence, PCR, ELISA, or virus isolation.
 
*The complement fixation test can be used to identify Eastern or Western EEV in infected mouse or chicken brains, cell culture fluid or amniotic-allantoic fluid
 
*The complement fixation test can be used to identify Eastern or Western EEV in infected mouse or chicken brains, cell culture fluid or amniotic-allantoic fluid
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*Virus isolation is the most definitive diagnostic method for EEE or WEE.  Brain is preferred, but virus has also been isolated from the liver and spleen. Samples of these tissues should be taken in duplicate, one set for virus isolation and the other placed in formalin for histopathology. Viral isolation specimens should be sent frozen unless they can be received refrigerated within 48 hours of sampling.  Unless clinical signs persist for more than 5days prior to death, EEE virus is frequently isolated from equine brain tissue.  WEE virus, however, is rarely isolated from tissues of infected horses.  Newborn mice, chicken embryos and a number of cell culture systems can be used for virus isolation.  Virus may also be isolated from cerebrospinal fluid (CSF) of acutely infected horses.
 
*Virus isolation is the most definitive diagnostic method for EEE or WEE.  Brain is preferred, but virus has also been isolated from the liver and spleen. Samples of these tissues should be taken in duplicate, one set for virus isolation and the other placed in formalin for histopathology. Viral isolation specimens should be sent frozen unless they can be received refrigerated within 48 hours of sampling.  Unless clinical signs persist for more than 5days prior to death, EEE virus is frequently isolated from equine brain tissue.  WEE virus, however, is rarely isolated from tissues of infected horses.  Newborn mice, chicken embryos and a number of cell culture systems can be used for virus isolation.  Virus may also be isolated from cerebrospinal fluid (CSF) of acutely infected horses.
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======Serology======
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====Serology====
    
Ab titre increases sharply within 24 hours of the initial viraemia, before clinical signs are apparent.  It then deteriorates over 6 months.  Samples taken when clinical signs appear are likely to miss the Ab peak and will demonstrate a decreasing titre.  Thus, serological confirmation of Eastern or Western EEV infection requires a four-fold or greater increase OR decrease in Ab titre in paired serum samples taken 10-14 days apart.  A presumptive diagnosis can be made on a single sample if an unvaccinated horse with suggestive clinical signs has Ab against only Eastern or Western EEV.  Colostral-derived Ab has a serum half-life of around 20days and may interfere with diagnosis in foals.
 
Ab titre increases sharply within 24 hours of the initial viraemia, before clinical signs are apparent.  It then deteriorates over 6 months.  Samples taken when clinical signs appear are likely to miss the Ab peak and will demonstrate a decreasing titre.  Thus, serological confirmation of Eastern or Western EEV infection requires a four-fold or greater increase OR decrease in Ab titre in paired serum samples taken 10-14 days apart.  A presumptive diagnosis can be made on a single sample if an unvaccinated horse with suggestive clinical signs has Ab against only Eastern or Western EEV.  Colostral-derived Ab has a serum half-life of around 20days and may interfere with diagnosis in foals.
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Most commonly, the PRN test or a combination of PRN and HAI tests is used to detect Ab against Eastern and Western EEV. Cross-reactivity occurs between Ab against these viruses in the CF and HAI tests.
 
Most commonly, the PRN test or a combination of PRN and HAI tests is used to detect Ab against Eastern and Western EEV. Cross-reactivity occurs between Ab against these viruses in the CF and HAI tests.
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======Clinical Pathology======
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====Clinical Pathology====
 
CSF samples demonstrate increased cellularity (50-400 mononuclear cells/µl) and protein concentration (100-200mg/dl).
 
CSF samples demonstrate increased cellularity (50-400 mononuclear cells/µl) and protein concentration (100-200mg/dl).
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=====(2) Post-mortem findings=====
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===Post-mortem findings===
 
'''''PRECAUTION'': infective viral particles may be present in CNS and other tissues'''.
 
'''''PRECAUTION'': infective viral particles may be present in CNS and other tissues'''.
 
Gross pathological lesions of the brain and spinal cord are rarely seen in horses, although traumatic ecchymotic haemorrhages and vascular congestion of the CNS may be evident. The extent of microscopic lesions is dictated by the severity of infection and duration of neurological involvement (Walton, 1981).  Such lesions with or without immunohistochemistry may be diagnostic.  The cerebral cortex, thalamus and hypothalamus are often severely affected.  Mononuclear meningitis, neuronal degeneration, diffuse and focal gliosis and perivascular cuffing are also seen.  Histological lesions of EEE are usually present throughout the CNS, with widespread and severe neutrophilic inflammation of the grey matter.  Lesions caused by Western EEV infection are more focal and lymphocytic in nature.  VEE cases often exhibit haemorrhage and liquefactive necrosis of the cerebral cortex, but lesions are not restricted to the CNS.  In the pancreas, acinar cells atrophy and duct cells undergo hyperplasia.  There may also be damage to the liver and heart.     
 
Gross pathological lesions of the brain and spinal cord are rarely seen in horses, although traumatic ecchymotic haemorrhages and vascular congestion of the CNS may be evident. The extent of microscopic lesions is dictated by the severity of infection and duration of neurological involvement (Walton, 1981).  Such lesions with or without immunohistochemistry may be diagnostic.  The cerebral cortex, thalamus and hypothalamus are often severely affected.  Mononuclear meningitis, neuronal degeneration, diffuse and focal gliosis and perivascular cuffing are also seen.  Histological lesions of EEE are usually present throughout the CNS, with widespread and severe neutrophilic inflammation of the grey matter.  Lesions caused by Western EEV infection are more focal and lymphocytic in nature.  VEE cases often exhibit haemorrhage and liquefactive necrosis of the cerebral cortex, but lesions are not restricted to the CNS.  In the pancreas, acinar cells atrophy and duct cells undergo hyperplasia.  There may also be damage to the liver and heart.     
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[[Image:Venezuelan_equine_encephalitis_virus.jpg|300px|thumb|right|''' (Photomicrograph of mouse brain tissue after dying of Venezuelan Encephalitis. Reveals neural necrosis and edema.  ''Sourced from Wikimedia Commons, Copyright of the Centers for Disease Control and Prevention (CDC) Public Health Image Library (PHIL) Image #2809, 2006)'' ''']]
 
[[Image:Venezuelan_equine_encephalitis_virus.jpg|300px|thumb|right|''' (Photomicrograph of mouse brain tissue after dying of Venezuelan Encephalitis. Reveals neural necrosis and edema.  ''Sourced from Wikimedia Commons, Copyright of the Centers for Disease Control and Prevention (CDC) Public Health Image Library (PHIL) Image #2809, 2006)'' ''']]
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====Differential diagnoses====
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==Differential diagnoses==
 
*Other togaviral encephalitides
 
*Other togaviral encephalitides
 
*Other [[Equine Viral Encephalitis|viral encephalitides]]
 
*Other [[Equine Viral Encephalitis|viral encephalitides]]
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*Toxicosis
 
*Toxicosis
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====Treatment====
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==Treatment==
 
No effective, specific treatment is available.  Supportive management includes:
 
No effective, specific treatment is available.  Supportive management includes:
 
*NSAIDs (phenylbutazone, flunixin meglumine) to control pyrexia, inflammation and discomfort
 
*NSAIDs (phenylbutazone, flunixin meglumine) to control pyrexia, inflammation and discomfort
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*Sling support for recumbent horses
 
*Sling support for recumbent horses
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====Prognosis====
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==Prognosis==
 
Comatose animals rarely survive.  Survivors exhibit functional improvement over weeks to months, but complete recovery from neurological deficits is rare.  Residual ataxia, depression and abnormal behaviour is often seen with EEE, but less so with WEE.  The mortality rates for neurological equine viral encephalitis are reportedly:
 
Comatose animals rarely survive.  Survivors exhibit functional improvement over weeks to months, but complete recovery from neurological deficits is rare.  Residual ataxia, depression and abnormal behaviour is often seen with EEE, but less so with WEE.  The mortality rates for neurological equine viral encephalitis are reportedly:
 
*EEE 75-100%
 
*EEE 75-100%
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It is generally assumed that infection does not provide protective immunity, however, protection for up to 2 years has been noted.
 
It is generally assumed that infection does not provide protective immunity, however, protection for up to 2 years has been noted.
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====Control====
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==Control==
=====(1) Vaccination=====
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=== Vaccination===
 
Most vaccines are killed (produced in cell culture and inactivated with formalin) and elicit significant increases in Ab titre after 3 days.  Protective titres last for 6-8 months.  Some cross-protection is seen between the serotypes but not between Western and Eastern EEV.  Monovalent, divalent and trivalent vaccines are available but the response to monovalent VEE vaccination is decreased in horses previously vaccinated against WEE and EEE.  The current recommendation is to vaccinate susceptible horses annually in late spring or several months before the high risk season.  Biannual or triannual vaccination should be employed in regions where the vector season is prolonged. Susceptible horses should also be vaccinated in the face of an outbreak.  Mares should be vaccinated one month prior to foaling to boost colostral-derived Ab, which persists for 6-7 months.  Although foals can be vaccinated at any time, early vaccination should be followed by boosters at 6 months and at one year.  Vaccination does not interfere with the ELISA assay for VEE.  '''''PRECAUTION'': human vaccination is recommended for vets in endemic areas'''.
 
Most vaccines are killed (produced in cell culture and inactivated with formalin) and elicit significant increases in Ab titre after 3 days.  Protective titres last for 6-8 months.  Some cross-protection is seen between the serotypes but not between Western and Eastern EEV.  Monovalent, divalent and trivalent vaccines are available but the response to monovalent VEE vaccination is decreased in horses previously vaccinated against WEE and EEE.  The current recommendation is to vaccinate susceptible horses annually in late spring or several months before the high risk season.  Biannual or triannual vaccination should be employed in regions where the vector season is prolonged. Susceptible horses should also be vaccinated in the face of an outbreak.  Mares should be vaccinated one month prior to foaling to boost colostral-derived Ab, which persists for 6-7 months.  Although foals can be vaccinated at any time, early vaccination should be followed by boosters at 6 months and at one year.  Vaccination does not interfere with the ELISA assay for VEE.  '''''PRECAUTION'': human vaccination is recommended for vets in endemic areas'''.
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=====(2) Vector control=====
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===Vector control===
 
Responsible use of insecticides and repellents, elimination of standing water, and stable screening will all help to reduce viral transmission.  Environmental application of insecticides may be useful in endemic areas or during an outbreak.  Horses infected with Venezuelan EEV should be isolated for 3 weeks after complete recovery.
 
Responsible use of insecticides and repellents, elimination of standing water, and stable screening will all help to reduce viral transmission.  Environmental application of insecticides may be useful in endemic areas or during an outbreak.  Horses infected with Venezuelan EEV should be isolated for 3 weeks after complete recovery.
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====References====
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==References==
 
Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) ''Equine Internal Medicine'', Third Edition, Saunders, Chapter 12.
 
Bertone, J.J (2010) Viral Encephalitis in Reed, S.M, Bayly, W.M. and Sellon, D.C (2010) ''Equine Internal Medicine'', Third Edition, Saunders, Chapter 12.
  
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