Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
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| Line 18: | Line 18: | ||
* Transvaginal Aspiration in the Mare: | * Transvaginal Aspiration in the Mare: | ||
| − | ** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum. | + | ** Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the [[Rectum_-_Anatomy_%26_Physiology|rectum]]. |
** Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina. | ** Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina. | ||
** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized. | ** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized. | ||
** Hypodermic needle is advanced through the vaginal wall into the antral follicle. | ** Hypodermic needle is advanced through the vaginal wall into the antral follicle. | ||
** Follicular fluid containing the oocyte is aspirated under constant vacuum. | ** Follicular fluid containing the oocyte is aspirated under constant vacuum. | ||
Revision as of 14:59, 16 July 2008
Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
- Direct follicle aspiration:
- A hyperdermic needle is inserted into the follicle.
- Follicular fluid is aspirated.
- Follicular fluid forcefully returned to the follicle.
- Repeated 2-3 times to dislodge oocytes.
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.