Difference between revisions of "In Vitro Fertilization - Anatomy & Physiology"
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* Donor is superovulated to provide large numbers of mature follicles. | * Donor is superovulated to provide large numbers of mature follicles. | ||
* Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content. | * Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content. | ||
− | * Oocytes captured in vitro. | + | * [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|Oocytes]] captured in vitro. |
Line 15: | Line 15: | ||
** Follicular fluid is aspirated. | ** Follicular fluid is aspirated. | ||
** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]]. | ** Follicular fluid forcefully returned to the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]]. | ||
− | ** Repeated 2-3 times to dislodge oocytes. | + | ** Repeated 2-3 times to dislodge [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocytes]]. |
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** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized. | ** Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the [[The_Ovary_-_Follicles_-_Anatomy_%26_Physiology|follicle]] can be visualized. | ||
** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle. | ** Hypodermic needle is advanced through the [[Female_Reproductive_Tract_-The_Vagina/Vestibule_-_Anatomy_%26_Physiology|vaginal wall]] into the antral follicle. | ||
− | ** Follicular fluid containing the oocyte is aspirated under constant vacuum. | + | ** Follicular fluid containing the [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocyte]] is aspirated under constant vacuum. |
− | * Aspirated oocytes are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_-_Anatomy_%26_Physiology#Capacitation|capacitated]] spermatozoa. | + | * Aspirated [[The_Ovary_-_Oocytes_-_Anatomy_%26_Physiology|oocytes]] are placed in a culture vessel with [[Copulation_-Sperm_in_the_Female_Tract_-_Anatomy_%26_Physiology#Capacitation|capacitated]] spermatozoa. |
** Could also be cultured in a 'nurse animal' in vivo. | ** Could also be cultured in a 'nurse animal' in vivo. | ||
* Embryos are incubated for a further period to the appropriate stage for transfer. | * Embryos are incubated for a further period to the appropriate stage for transfer. |
Revision as of 09:45, 17 July 2008
Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
- Oocytes captured in vitro.
- Direct follicle aspiration:
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.
- Aspirated oocytes are placed in a culture vessel with capacitated spermatozoa.
- Could also be cultured in a 'nurse animal' in vivo.
- Embryos are incubated for a further period to the appropriate stage for transfer.
- Embryos transferred to recipient female.
- Can be transferred at the correct stage of the recipient's oestrous cycle.
- Embryos transferred around the blastocyst stage.
- Success rates are improving.
- Embryos can be frozen, eliminating the need for tight synchronisation. This allows them to be shipped to other locations.
- Increases incidence of large offspring syndrome.