In Vitro Fertilization - Anatomy & Physiology
Jump to navigation
Jump to search
BACK TO REPRODUCTIVE SYSTEM
BACK TO REPRODUCTIVE TECHNOLOGIES
Oocyte Collection
- Donor is superovulated to provide large numbers of mature follicles.
- Pre-ovulatory follicles collected by surgical procedure involving aspiration of the follicular content.
- Direct follicle aspiration:
- A hyperdermic needle is inserted into the follicle.
- Follicular fluid is aspirated.
- Follicular fluid forcefully returned to the follicle.
- Repeated 2-3 times to dislodge oocytes.
- Transvaginal Aspiration in the Mare:
- Prior to the procedure, mares are injected with propatheline bromide (sedative) to relax the rectum.
- Lubricated ultrasound transducer is inserted into the vagina and held in the fornix vagina.
- Ovary transrectally positioned against the dorsal vaginal wall directly over the transducer head so that the follicle can be visualized.
- Hypodermic needle is advanced through the vaginal wall into the antral follicle.
- Follicular fluid containing the oocyte is aspirated under constant vacuum.