Changes

*''Tissue containing bone'' - Tissues containing bone should be formalin-fixed then treated with a decalcifying solution.

*''Tissue containing bone'' - Tissues containing bone should be formalin-fixed then treated with a decalcifying solution.

*''Tissue for virus isolation'' - For viral isolation, place tissue sections in individual sterile bags and freeze.

*''Tissue for virus isolation'' - For viral isolation, place tissue sections in individual sterile bags and freeze.

===Bone marrow from snakes==

===Bone marrow from snakes===

Bone marrow from snakes is difficult but not impossible to obtain from snakes. The bone marrow cavity of the ribs of large snakes will yield a sample. The area around the dorsal fourth of a rib is aseptically prepared. The skin is incised and the cortical bone of the rib is drilled through with a 21- to 23-gauge spinal needle. The needle is then advanced a few millimetres to the marrow cavity. For smaller species, surgical removal of a portion of a rib or vertebral spinous process is possible. It is fixed in formal saline and placed in a decalcifying solution until soft enough to allow histological processing. It is also possible to obtain marrow from the ventral vertebral bodies by biopsy.

Bone marrow from snakes is difficult but not impossible to obtain from snakes. The bone marrow cavity of the ribs of large snakes will yield a sample. The area around the dorsal fourth of a rib is aseptically prepared. The skin is incised and the cortical bone of the rib is drilled through with a 21- to 23-gauge spinal needle. The needle is then advanced a few millimetres to the marrow cavity. For smaller species, surgical removal of a portion of a rib or vertebral spinous process is possible. It is fixed in formal saline and placed in a decalcifying solution until soft enough to allow histological processing. It is also possible to obtain marrow from the ventral vertebral bodies by biopsy.

==Swab==

==Swab==