The horse should be starved for 18 - 24 hours prior to the test and then administered 1g / kg of D-glucose via a [[Nasogastric intubation in the horse|nasogastric tube]]. Blood samples to measure the glucose concentraions should be collected at 0,30,60,90,120,150,180,210 and 240 minutes after the carbohydrate has been given. More samples after 6 hours should be taken if the results are of questionable significance. The blood samples should be put into sodium flouride tubes to measure the glucose. Sedation and prolonged fasting prior to the test can alter the rate of absorption of glucose and the gastrointestinal transit times and so should be avoided to obtain reliable results. A glucose curve should be created to assist the interpretation of the results. | The horse should be starved for 18 - 24 hours prior to the test and then administered 1g / kg of D-glucose via a [[Nasogastric intubation in the horse|nasogastric tube]]. Blood samples to measure the glucose concentraions should be collected at 0,30,60,90,120,150,180,210 and 240 minutes after the carbohydrate has been given. More samples after 6 hours should be taken if the results are of questionable significance. The blood samples should be put into sodium flouride tubes to measure the glucose. Sedation and prolonged fasting prior to the test can alter the rate of absorption of glucose and the gastrointestinal transit times and so should be avoided to obtain reliable results. A glucose curve should be created to assist the interpretation of the results. |